基于聚铁氰酸钴/苝四甲酸二酐衍生物复合膜固定辣根过氧化物酶生物传感器的研究  

A Horseradish Peroxidase Biosensor Based on Cobalt Hexacyanoferrate and Derivative of 3,4,9,10-Perylenetetracarboxylic Dianhydride Composite Film

作　　者：李华刚[1,2,3] 袁若[1,2] 柴雅琴[1,2] 苗向敏[1,2] 卓颖[1,2] 洪成林[1,2] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]发光与实时分析教育部重点实验室,重庆400715 [3]遵义师范学院化学系,贵州遵义563002

出　　处：《西南大学学报（自然科学版）》2009年第9期97-101,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20675064);重庆市自然科学基金资助项目(CSTC-2005BB4100)

摘　　要：采用循环伏安（CV）法在金电极表面电聚合一层铁氰酸钴（CoHCF），利用花四甲酸二酐衍生物（PTC-NH2）成膜能力强又带有许多氨基功能团的特点将其固定在CoHCF表面，再通过静电吸附和共价键合作用将纳米金和辣根过氧化物酶（HRP）的复合物制得过氧化氢生物传感器VHRP-Au／PTC-NH2／CoHCF／Au]．考察了该传感器对过氧化氢的电化学特性，实验表明，该传感器具有良好的生物催化活性，响应电流与过氧化氢浓度在2．0×10^-6-1．6×10^-3mol／L范围呈良好的线性关系，检出限为7．28×10^-7mol／L．The cobalt hexacyanoferrate （CoHCF） film modified-gold electrode was successfully prepared by cyclic voltammetry （CV）. Then, the derivative of 3,4,9,10-perylenetetracarboxylic dianhydride （PTCNH2）, which contains rich amino groups and owns good film-forming properties, was dropped on the surface of CoHCF. Finally, the composite of nano-gold and horseradish peroxidase （HRP） was immobilized on PTC-NH2 surface via the effect of electrostatic adsorption and covalent bonding to construct a new H2O2 biosensor, denoted as HRP-Au/PTC-NH2/CoHCF/Au electrode. The electrocatalytic behavior of the modified electrode to H2O2 was investigated by cyclic voltammetry and chronoamperometry. The experiment results showed that the modified electrode had excellent biocatalytic activity for the reduction of H2O2. The linear range of this biosensor was 2.0×10^-6-1.6× 10^-3 mol/L with a detection limit of 7.28× 10^-7 mol/L.

关 键 词：铁氰酸钴 藐四甲酸二酐衍生物 纳米金 辣根过氧化物酶 生物传感器 

分 类 号：O657.1[理学—分析化学]