传染性囊病变异株病毒BK912的培育及鉴定  

Culture and Characterization of Infectious Bursal Disease Virus Variant BK912 Strain

作　　者：刘有昌[1] 刘爵[1] 周蛟[1] 姚炜光[1] 范书曦[1] 张方亮[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所

出　　处：《华北农学报》1998年第3期132-136,共5页Acta Agriculturae Boreali-Sinica

摘　　要：用由国外引进的ＩＢＤ变异株鸡胚适应毒经ＣＥＦ传代适应后所获得的ＢＫ９１２Ｆ５毒液进行了病毒理化特性试验和形态学观察，并与标准Ｉ型毒ＣＪ８０１ＢＫＦ株进行了抗原相关性检测。实验证明，ＢＫ９１２具有ＩＢＤＶ的基本特性且与标准Ｉ型毒ＣＪ８０１ＢＫＦ株处于不同血清亚型。以２０００ＴＣＩＤ５０／０．２ｍｌ的ＢＫ９１２冻干活疫苗免疫ＳＰＦ鸡后能完全抵抗美国标准变异株强毒１０８４Ａ的攻击。一系列试验证明ＢＫ９１２仍为ＩＢＤＶ变异株毒。The IBDV strain BK912F 5 was obtained from imported chicken embryos adapted variant virus through CEF adaptation and it was characterized and observed for physiochemical and morphological properties respectively. The antigenic relation between BK912 and standard serotype I CJ801BKF strain was also tested. The results showed that the BK912 strain possessed basic properites of IBDV and belonged to a different subtype compared with CJ801BKF strain. The SPF chickens vaccinated with lyophilized live BK912 vaccine at 2000TCID 50 /0 2ml each bird, could protect the challenge of USA virulent variant completely. A series of tests fully proved that the BK912 was still an IBDV variant strain.

关 键 词：鸡病 传染性囊病 BK912毒株 变异株 培育 鉴定 

分 类 号：S858.31[农业科学—临床兽医学]