干扰素α对Ⅰ型胶原及转化生长因子β1基因表达的影响  

Effects of IFN alpha on the expressions of Collagen I and transforming growth factor beta 1 in hepatic stellate cell activated by PDGF-BB

作　　者：胡静[1] 李智伟[1] 杨秀锦[1] 杨悦杰[1] 马力[1] 

机构地区：[1]中国医科大学附属盛京医院感染科,沈阳110003

出　　处：《中华肝脏病杂志》2009年第9期657-660,共4页Chinese Journal of Hepatology

基　　金：辽宁省教育厅科学基金课题（20060979）

摘　　要：目的观察干扰素α（IFNα）对血小板衍生生长因子-BB（PDGF-BB）刺激的大鼠肝星状细胞（HSC）分泌Ⅰ型胶原及转化生长因子β1（TGFβ1）基因表达的影响，探讨IFNα抗肝纤维化的可能机制。方法体外培养大鼠HSC系rHSC-99，分别用0，0．0125、0．0250、0．0500，0．1000、0．2000、0．4000ng／ml IFNα、PDGF—BB干预和两者共同干预，用四甲基偶氮唑盐实验观察各组对HSC细胞活力的影响，采用逆转录聚合酶链反应方法测定各组对HSC细胞Ⅰ型胶原mRNA和TGFβ1 mRNA表达的影响。结果（1）HSC细胞活力（A值）PDGF—BB干预组为1．35±0．22，空白对照组为0．89±0．12，两组比较，F=16．311，P〈0．05，差异有统计学意义，说明PDGF-BB可提高HSC细胞活力。0．025、0．050、0．100、0．2000、0．400ng／ml IFNα加PDGF-BB共干预组，4值分别为0．84±0．18、0．45±0．15、0．26±0．01、0．33±0．07、0．30±0．06，较空白对照组明显降低，F=7．430，P〈0．05，差异有统计学意义，说明IFNα与PDGF-BB共同作用可抑制HSC细胞活力，且在0．025～0．100ng／ml范围内随着IFNα浓度的增加其抑制作用越明显。（2）0．050、0．100、0．200ng／ml IFNα加PDGF—BB共干预各组Ⅰ型胶原mRNA相对表达值分别为0．94±0．19、0．61±0．12、0．52±0．02，空白对照组为1．41±0．01，共干预各组比空白对照组均明显降低，F=127．921，P〈0．05，差异有统计学意义。0．050、0．100、0．200ng／ml IFNα加PDGF—BB共干预组各组TGFβ1 mRNA相对表达值分别为1．18±0．06、1．15±0．10、1．39±0．04，空白对照组为1．62±0．12，共干预各组比空白对照组均明显降低，F=82．115，P〈0．05，差异有统计学意义，说明IFN仅与PDGF-BB共同作用对HSC细胞Ⅰ型胶原、TGFβ1基因的表达有抑制作用，且随着浓度的增加其抑制作用越明显。结论IFNα对PDGF—BB诱导的HSC细胞活力及Ⅰ型胶原Objective To investigate the effect of IFN alpha on the expressions of Collagen Iand TGFA1 in hepatic stellate cell activated by PDGF-BB. Methods Hepatic stellate cells （rHSC-99） treated with IFN alpha of different concentration （0, 0.0125, 0.025, 0.050, 0.100, 0.200, 0.400 ng/ml）. The cell viability of HSC was measured by MTT. The levels of Col-I mRNA and TGF β 1 mRNA were measured by the quantitative reverse-transcription polymerase chain reaction （RT-PCR）. Results （1） When HSC was exposed in PDGF-BB, the cell viability of HSC （1.35 ± 0.22） was higher than that of the control group （0.890 ± 0.12） （F = 16.311, P 〈 0.05）, indicating that PDGF-BB can promote the cell viability of HSC. When HSC was exposed to both PDGF-BB and different concentration of IFN alpha （0.025, 0.05, 0.1, 0.2, 0.4 ng/ml）, the cell viability of HSC （0.840 ± 0.18, 0.450 ± 0.15, 0.260 ± 0.01, 0.330 ± 0.07, 0.30 ± 0.06） were lower than that of the control group （0.890 ± 0.12） （F = 7.430, P 〈 0.05）, indicating that the cell viability of HSC was inhibited when HSC was exposed to both PDGF-BB and different concentrations of IFN alpha. Furthermore, within the range of 0.025 ng/ml to 0.1 ng/ml, the effect of IFN alpha was dosedependant. （2）. The relative expression values of Col-I mRNA in different groups of （0.05, 0.1, 0.2 ng/ml） IFN alpha ＋PDGF-BB are （0.940 ± 0.19, 0.610 ± 0.12, 0.520 ± 0.02）, which were lower than those in the control group （1.410 ± 0.0l） （F = 127.921, P 〈 0.05）. The relative expression values ofTGF β 1 mRNA in different groups of （0.05, 0.1, 0.2 ng/ml） IFN alpha ＋PDGF-BB are （1.180 ± 0.06, 1.150 ± 0.10, 1.390 ± 0.04）, again were lower than those in the control group （1.620 ± 0.12） （F = 82.115, P 〈 0.05）. These results indicated that the expression of Col-I mRNA and TGF β1 mRNA was remarkably inhibited when HSC was exposed in both PDGF-BB and IFN alpha. Conclusion The cell viability of HSC and the expression of Coi-I m

关 键 词：肝硬化 干扰素Α 血小板衍生生长因子-β 胶原 Ⅰ型 转化生长因子Β 肝星状细胞 

分 类 号：R285.5[医药卫生—中药学] R575.2[医药卫生—中医学]