脊髓损伤局部表达NgR的巨噬细胞的体外分离培养  

The separation and culture of macrophages expressed with NgR in vitro from spinal cord injury rats

作　　者：尹国栋[1] 倪斌[1] 周风金[1] 杨军[1] 郭翔[1] 杨健[1] 刘军[1] 

机构地区：[1]第二军医大学附属长征医院骨科,上海200003

出　　处：《脊柱外科杂志》2009年第4期198-200,共3页Journal of Spinal Surgery

基　　金：国家自然科学基金资助项目(30872612)

摘　　要：目的从脊髓损伤局部分离培养巨噬细胞,并鉴定其表达Nogo受体(Nogo receptors,NgR)情况。方法胰酶消化大鼠损伤脊髓组织获取其脊髓损伤局部巨噬细胞,细胞贴壁后用添加5%胎牛血清的RPM I 1640培养,显微镜下观察巨噬细胞形态及生物学特点,免疫荧光化学方法鉴定其是否表达CD68及NgR。结果原代培养的巨噬细胞贴壁生长,细胞形态呈圆形、椭圆形等,可存活约3周,表现为CD68抗原阳性,多数细胞NgR抗原阳性。结论成功地从脊髓损伤局部分离出巨噬细胞,多数细胞表达NgR抗原。Objective To separate and culture the macrophages expressed with NgR spinal cord injury rats in vitro.Methods Trypsin was applied to harvest the maerophages from the isolated traumatic spinal cord tissues. After adherence the macrophages were cultured in RPMI 1640 medium added with 5% fetal calf serum. The morphological and biological feature were observed in microscope. Immunochemistry staining was used to prove the characteristic of macrophages which expressed NgR and CD68. Results Most of the primary macrophages presented round and elliptic shape after adherence. The macrophages could survive for about 3 weeks and be marked by CD68 antibody and NgR antibody for majority of the cells. Conclusion The macrophages are isolated from the traumatic spinal cord tissues successfully and most of them are presented with NgR antigen.

关 键 词：脊髓损伤 巨噬细胞 细胞培养技术 荧光抗体技术 

分 类 号：R651.21[医药卫生—外科学]