海南部分荔枝种质资源亲缘关系的SSR分析  

Analysis of Genetic Relationship of Hainan Litchi Germplasm Resources by SSR Marker

作　　者：姚庆荣[1] 赵长增[2] 王文泉[3] 

机构地区：[1]甘肃行政学院,兰州730010 [2]甘肃农业大学农学院,兰州730070 [3]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《植物研究》2009年第5期628-632,共5页Bulletin of Botanical Research

摘　　要：利用SSR标记对22份荔枝材料进行了亲缘关系分析,从32对引物中筛选出22对多态性引物用于荔枝SSR扩增,共扩增到52条带,其中多态性条带49条,多态性百分率为94.23%。多态性条带经POPGENE32软件统计分析表明,22个位点的平均有效等位基因频率(Ne)、平均基因杂合度(H)、平均Shannon遗传多样性指数(Hi′)分别为1.364 3、0.296 0、0.417 0。通过NTSYS聚类结果显示,在相似系数为0.51处,供试材料被聚为两大类,第一类包括13份材料,又可分为两个亚类,第二类包括9份材料。22 Litchi germplasm were used as materials for analyzing their genome polymorphism by SSR markers. 22 primers selected from 32 primers were used for SSR amplification. A total of 52 bands were generated, of which 49 bands were polymorphic bands. The percentage of polymorphic band （PPB） was 94.23%. According to the analysis results of POPGENE32 software, the average effective number of alleles（ Ne）, the Nei＇s average gene diversity （H） and the average of Shannon＇ s genetic diversity （ H＇i ） index was 1. 364 3, 0.2960 and 0.4170, respectively. The UPGAM dendrogram gotten by NTNSYS cluster analysis implied that 22 Litchi germplasm resources could be divided into 2 groups on the basis of genetic distance 0.51. The fist group included 13 cultivars. The second group included 9 cuhivars.

关 键 词：荔枝 亲缘关系 SSR 海南 

分 类 号：S667.1[农业科学—果树学]