系统性红斑狼疮中医证型与IL-10 mRNA、IL-18 mRNA及Fas mRNA表达水平的相关性研究  

Relationship between Chinese Medicine Syndrome Type and mRNA Expressions of IL-10,IL-18 and Fas in Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus

作　　者：张峻岭[1,2] 宫泽琨[3] 

机构地区：[1]天津大学化工学院,天津300072 [2]天津市长征医院皮肤科 [3]天津市儿童医院皮肤科

出　　处：《中国中西医结合杂志》2009年第9期783-786,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：天津市卫生局中医;中西医结合科研基金资助项目(No.200029)

摘　　要：目的观察系统性红斑狼疮(systemic lupus erythematosus,SLE)各中医证型患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中白细胞介素10(IL-10)mRNA、白细胞介素18(IL-18)mRNA、Fas mRNA的表达量,以探求SLE中医辨证分型的客观化参考指标。方法根据中医辨证将SLE分为5种证型,选择常见的4种证型,即气血热盛型(12例)、脾肾阳虚型(19例)、气阴两虚血瘀型(23例)、气滞血瘀肝郁型(28例),应用SYBR GreenⅠ实时荧光定量RT-PCR技术,检测并比较不同证型SLE患者及正常对照组PBMCs中IL-10 mRNA、IL-18 mRNA、Fas mRNA表达的差异。结果SLE患者PBMCs IL-10、IL-18、Fas mRNA的表达量(分别为:0.40±0.53,1.41±1.93,0.33±0.70)明显高于正常对照组(分别为:0.07±0.11,0.24±0.25,0.08±0.21)(P<0.01,P<0.05);3种细胞因子的mRNA表达量从气血热盛型、脾肾阳虚型、气阴两虚血瘀型到气滞血瘀肝郁型依次递减,其中气血热盛型、脾肾阳虚型明显高于气阴两虚血瘀型和气滞血瘀肝郁型(P<0.01),气血热盛型与脾肾阳虚型比较,差异无统计学意义(P>0.05),气阴两虚血瘀型与气滞血瘀肝郁型比较,差异无统计学意义(P>0.05)。结论IL-10 mR-NA、IL-18 mRNA、Fas mRNA表达水平可以作为SLE中医辨证分型的客观化参考指标。Objective To explore the mRNA expressions of IL-10,IL-18 and Fas in peripheral blood mononuclear cells（PBMCs） in systemic lupus erythematosus（SLE） patients of various Chinese medicine syndrome types in order to seek the objective indices for syndrome differentiation of SLE.Methods SLE patients were sorted into various syndrome types by Chinese medicine syndrome differentiation.In them,patients with the 4 often encountered types were selected,i.e.the qi-xue excessive heat type（Group A,12 patients）,the Pi-Shen yang-deficiency type（Group B,19 patients）,the qi-yin deficiency with blood stasis type（Group C,23 patients）,and the qi stagnation and blood stasis with gan stagnation type（Group D,28 patients）.The mRNA expressions of IL-10,IL-18 and Fas in PBMCs were detected by SYBR GreenⅠreal time PCR.Results The mRNA expressions of IL-10,IL-18 and Fas in PBMC of all SLE patients（0.40±0.53,1.41±1.93 and 0.33±0.70） were significantly higher than those in the normal control group（0.07±0.11,0.24±0.25,0.08±0.21,P〈0.01,P〈0.05）,their levels in different syndrome types decreased in order of type A→B→C→D.The levels of the three indices in patients of type A and B were obviously higher than those in patients of type C and D（P〈0.01）,while no significant difference was found between type A and B（P〉0.05）,and between type C and D（P〉0.05）.Conclusion The mRNA expressions of IL-10,IL-18 and Fas in PBMCs could be taken as the objective indices for reference in syndrome differentiation of SLE.

关 键 词：系统型红斑狼疮 辨证分型 白细胞介素10 白细胞介素18 Fas 

分 类 号：R259[医药卫生—中西医结合]