胸腺肽-β4在小鼠卵母细胞和早期胚胎中的表达与分布规律  

Expression and Distribution of Thymosin-β4 in Mouse Oocytes and Early Embryos

作　　者：张岩[1] 李树峰[1] 杨彩荣[1] 郑可佳[1] 李宁[1] 严云勤[1] 

机构地区：[1]东北农业大学动物细胞与发育生物学教研室,哈尔滨150030

出　　处：《生物化学与生物物理进展》2009年第9期1193-1201,共9页Progress In Biochemistry and Biophysics

摘　　要：胸腺肽-β4(thymosin-β4,Tb4)是一种重要的G-actin遮蔽因子(G-actin sequestering factor),在细胞微丝活动中有着调节G-actin活性的作用.以往报道证实了Tb4在细胞中具有广泛的生理作用,但其在哺乳动物卵母细胞成熟和早期胚胎发育等方面的作用,迄今还没有系统的研究报道.以昆明白小鼠卵巢、卵母细胞和早期胚胎作为实验材料,以免疫(荧光)组织化学和RT-PCR技术为主要研究方法,对Tb4的表达与分布进行了研究.结果显示,Tb4在相关发育过程中,存在差异性的表达和定位变化.结果表明,在小鼠卵母细胞成熟和早期胚胎的发育过程,Tb4能够通过表达与分布的变化对细胞微丝活动和细胞增殖活动进行调控,对小鼠卵母细胞成熟、早期胚胎发育以及胚胎着床过程有重要的作用.Thymosin-β4 （thymosin-β4, Tb4） is an important G-actin sequestering factor in cells, which could regulate the activity of G-actin in various mierofilaments events. Previously, many researches have reported that Tb4 has broad cellular and physiological functions, but until now, there is lack of systematic research on mammal oocytes and early embryonic development. Tb4 is a small hydrophilic peptide, initially, which separated and purified from bovine thymus component V, with a molecular mass of 5 ku, and compose of 43 amino acid residues. Tb4 molecule is highly conservative, which has been founded in a variety of tissues and organs in vertebrates, and also exists in part invertebrates. This peptide has extensive biological functions. Recent studies have shown that it closely relate with many physiological and pathological activities, such as immune regulation, neurological development, wound healing, inflammatory response, angiogenesis, apoptosis, tumor occurrence and migration. Dynamic events of microfilaments in cells always accompany with G-actin activity, while Tb4 could bind G-actin with a ratio of 1 ： 1 to inhibit G-actin to polymerize into microfilaments. In cells, nearly half of G-actin are bound with various of sequestering factors, which effects on both G-actin activity regulation and its reservation, while Tb4 is the chief executer of G-actin sequestering factor family. The ovaries, oocytes and early embryos of Kunming mouse was used to investigate the expression and distribution of Tb4 in oocytes and eary embryos. During the experiment, the irnmuno-histochemistry, immuno-fluorescence-histochemistry and RT-PCR technique were utilized as chief study methods. The discipline of Tb4 expression and distribution in oocytes maturation and early embryonic development were studied both in vivo and vitro. The results showed that Tb4 were different in expression and localization during mouse oocytes maturation and early embryonic development. Immuno-histochemistry results illustrated that, accompany with f

关 键 词：胸腺肽-β4 小鼠 卵母细胞 胚胎 微丝 免疫荧光组织化学 激光共聚焦扫描显微镜 

分 类 号：Q2[生物学—细胞生物学]