输注供者CD8^＋CD28^-调节性T淋巴细胞抑制大鼠肝移植急性排斥反应的作用  

The suppressive effect of CD8^＋CD28^- regulatory T cells from spontaneous tolerance models on the acute rejection responses in rat liver transplantation

作　　者：陈宁[1] 陈国栋[1] 尤鹏[1] 刘玉兰[1] 

机构地区：[1]北京大学人民医院消化内科,100044

出　　处：《中华器官移植杂志》2009年第9期524-526,共3页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金（30471629）

摘　　要：目的评价具有免疫抑制作用的CD8^＋CD28^-调节性T淋巴细胞（Treg）体内输注在抑制大鼠肝移植急性排斥反应中的作用。方法建立近交系大鼠肝移植自发耐受及急性排斥反应模型。从肝移植自发耐受模型受者脾脏中分离CD8^＋CD28^- Treg，于急性排斥反应模型建立前1d输注给受者，比较不同的输注组问受者的存活时间和移植肝病理学表现。结果来自自发耐受模型（LEW大鼠为供者，DA大鼠为受者）的CD8^＋CD28^- Treg输注可以延长急性排斥反应模型（LEW大鼠为供者，BN大鼠为受者）受者的存活时间，由（14．0±2．2）d延长至（24．0±3．0）d（P〈0．01），移植肝病理学显示排斥反应程度减轻。结论大鼠肝移植自发耐受模型受者体内诱导的CD8^＋CD28^- Treg具有抑制急性排斥反应的作用，该免疫抑制作用具有抗原特异性。Objective To evaluate the suppressive effect of CD8^＋CD28^- regulatory T cells （Treg） in vivo from spontaneous tolerance models on the acute rejection responses in rat liver transplantation. Methods Spontaneous tolerance models of inbred rat liver transplantation were established. CD8^＋CD28^- Treg isolated from recipients of spontaneous tolerance models were adoptively transferred into the recipients with acute rejection responses one day before the operation. The survival time and histological changes were observed in the adoptive-transferred models. Results CD8^＋CD28^- Treg from spontaneous tolerance models （LEW→DA） prolonged the survival time of the recipients in acute rejection models （LEW→BN） （from 14. 0 ± 2. 2 days to 24. 0 ± 3. 0 days, P = 0. 0049）, and the severity of rejection was alleviated in liver pathology. Conclusion CD8^＋ CD28^ - Treg from spontaneous tolerance models can suppress the acute rejection responses in rat liver transplantation,with antigen-specific properties.

关 键 词：肝移植 T淋巴细胞 调节性 急性排斥 

分 类 号：R686[医药卫生—骨科学]