水分胁迫对不同基因型小麦幼芽蛋白质表达和某些生理特性的影响  

Effects of water stress on protein expression and physiological properties of different genotype wheat (Triticum aestivum L.) sprouts

作　　者：戴明[1] 邓西平[2] 杨淑慎[1] 曹让[1] 郭宏波[1] 张芳[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]中国科学院水利部水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌712100

出　　处：《应用生态学报》2009年第9期2149-2156,共8页Chinese Journal of Applied Ecology

基　　金：陕西省自然科学基金项目(2007C117);黄土高原土壤侵蚀与旱地农业国家重点实验室专项(10502)资助

摘　　要：以抗旱小麦品种长武134和不抗旱小麦品种郑引1号为试材,采用-1.2MPaPEG6000处理种子,研究不同水分条件下小麦幼芽中蛋白质表达和部分生理特性的变化.聚丙烯酰胺凝胶电泳结果表明:水分胁迫可诱导抗旱品种幼芽产生分子量约39.5kDa和23.0kDa两种新蛋白亚基,不抗旱品种则无新亚基产生;在正常供水条件下,随着生育期延长,两品种中分子量为48.5kDa的蛋白亚基表达量逐渐增强,因其对水分敏感且属于新发现蛋白,初步命名为水敏感蛋白.生理特性测定结果表明,水分胁迫条件下长武134的根芽比和相对含水量均高于郑引1号,而细胞膜相对透性和丙二醛含量则低于郑引1号.With drought-resistant wheat （ Triticum aestivum） cuhivar Changwu 134 and droughtsensitive cultivar Zhengyin 1 as test materials, and by using - 1.2 MPa PEG 6000 to treat their seeds, this paper studied the protein expression and physiological properties of the sprouts under different soil moisture conditions. SDS-PAGE analysis showed that water stress induced the production of two new proteins with molecular weights of 39. 5 kDa and 23.0 kDa in Changwu 134 but not in Zhengyin 1 sprouts. Under normal water supply, the expression of the protein with molecular weight of 48.5 kDa in the sprouts of both Changwu 134 and Zhengyin 1 increased with sprout growth. This protein was preliminarily named as water-sensitive protein, due to its water-sensitivity and of newly discovered protein. The determinations of physiological properties showed that under water stress, the sprouts of drought-resistant Changwu 134 had higher root/shoot ratio and higher relative water content, but lower relative membrane permeability and lower malondlaldehyde content than those of drought-sensitive Zhengyin 1.

关 键 词：小麦 水分胁迫 蛋白表达 生理特性 

分 类 号：S512.1[农业科学—作物学]