苦瓜蛋白逆转K562/AO2细胞多药耐药性的研究  

Study on reversal of multidrug resistance in K562/AO2 cells by momordica

作　　者：叶爱芳[1] 尹丽慧[1] 熊术道[1] 韩义香[1] 章圣辉[1] 吴建波[1] 

机构地区：[1]温州医学院第一附属医院内科实验室

出　　处：《中国临床药理学与治疗学》2009年第7期766-769,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省中医药青年基金项目(2006Y013)

摘　　要：目的:研究非细胞毒性质量浓度苦瓜蛋白对耐阿霉素的人红白血病细胞株K562/AO2多药耐药性的逆转作用和促凋亡作用。方法:采用CCK-8法测定苦瓜蛋白的细胞毒性及其对K562/AO2细胞敏感性的影响,用流式细胞仪检测K562/AO2细胞经不同药物处理后细胞的凋亡情况。结果:苦瓜蛋白对K562/AO2细胞有一定的细胞毒作用,其非细胞毒性质量浓度为5μg/mL,非细胞毒性质量浓度苦瓜蛋白对K562/AO2细胞对阿霉素、长春新碱(VCR)和柔红霉素的耐药性都有部分逆转作用(分别为5.4、6.5和4.0倍);5μg/mL苦瓜蛋白联合VCR诱导K562/AO2细胞凋亡,凋亡率为(19.38±1.06)%,而对照组为(1.64±0.27)%,单一苦瓜蛋白组为(3.79±0.82)%,单一VCR组为(9.83±0.98)%。结论:苦瓜蛋白能部分逆转人红白血病K562/AO2细胞对阿霉素、VCR和柔红霉素的耐药,一定剂量的苦瓜蛋白与VCR联合应用可增加肿瘤细胞凋亡率。AIM： To study the reverse and apoptosis effects of momordica at non-cytotoxic concentration reversing the muhidrug resistance （MDR） leukemia cell line K562/AO2. METHODS：The cytotoxic effect of momordica and the sensitivity to the K562/AO2 cell lines were determined by CCK-8 colorimetry. The apoptosis rate of the K562/AO2 cell lines in control group and other groups were detected by flow cytometry. RESULTS： Momordica had definite cytotoxic effect on K562/AO2, the non-cytotoxic concentration was 5 μg/ mL, the momordica at non-cytotoxic concentration could partly reverse the MDR of K562/AO2 cell lines to adriamycin, vincristine, daunomycin （5.4, 6.5 and 4.0 times）. The apoptosis of the K562/AO2 cell lines were induced at the 5 μg/mL momordica combined with vincristine, and the apoptosis rate was （19.38 ± 1.06） %. The apoptosis rate of the control group, single momordica group, single vincristine group were （1.64 ± 0.27）%, （3.79 ± 0.82）%, （9.83 ± 0.98 ） %, respectively. CONCLUSION： Momordica could partly reverse the MDR of K562/AO2 cell lines in different groups such as adriamycin, vincristine and daunomycin. A certain dose of momordica combined with vincristine could increase the apoptosis rate of tumour cells.

关 键 词：苦瓜蛋白 K562/AO2细胞 多药耐药 

分 类 号：R28[医药卫生—中药学]