Prostate cancer targeted MRI nanoprobe based on superparamagnetic iron oxide and copolymer of poly(ethylene glycol) and polyethyleneimin  

作　　者：ZHOU JianHua HUANG Lu WANG WeiWei PANG Jun ZOU Yang SHUAI XinTao GAO Xin 

机构地区：[1]Department of Urology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China [2]BME Center, Schoolof Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China [3]Department of Radiology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China

出　　处：《Chinese Science Bulletin》2009年第18期3137-3146,共10页

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 30772178, 50673103);Natural Science Foundation of Guangdong Province (Grant Nos. 7117362, 7003703);Key Clinical Program of Ministry of Health Foundation of China

摘　　要：Methoxyl poly (ethylene glycol) (mPEG-OH) was successfully grafted onto branched polyethyleneimine (hy-PEI) to yield a water soluble graft copolymer mPEG-g-PEI. This copolymer may package super- paramagnetic iron oxide (SPIO) by ligand exchange. The SPIO weight percentage in the polymer coated nanoparticles was determined to be 55%, the size and zeta potential of nanoparticles was 50 nm and 12 mV respectively. Antibody fixation onto the complex (mPEI-g-PEG-SPIO) surface layer was achieved by activated single chain monoclonal antibody against prostate stem cell antigen (PSCA). Our study showed that the single chain antibody functionalized nanoprobe (scAbPSCA-PEI-g-PEG-SPIO) with a small size can specifically enter the prostate cancer cells, decreasing MRI T2-weighted signal intensity of prostate cancer cells to 44.76%. Our results revealed that the potential of this magnetic nanoparticulate system promised as a novel MRI nanoprobe for early diagnosis of prostate cancer (PCa).Methoxyl poly （ethylene glycol） （mPEG-OH） was successfully grafted onto branched polyethyleneimine （hy-PEI） to yield a water soluble graft copolymer mPEG-g-PEI. This copolymer may package superparamagnetic iron oxide （SPIO） by ligand exchange. The SPIO weight percentage in the polymer coated nanoparticles was determined to be 55%, the size and zeta potential of nanoparticles was 50 nm and 12 mV respectively. Antibody fixation onto the complex （mPEI-g-PEG-SPIO） surface layer was achieved by activated single chain monoclonal antibody against prostate stem cell antigen （PSCA）. Our study showed that the single chain antibody functionalized nanoprobe （scAbpscA-PEI-g-PEG-SPIO） with a small size can specifically enter the prostate cancer cells, decreasing MRI T2-weighted signal intensity of prostate cancer cells to 44.76%, Our results revealed that the potential of this magnetic nanoparticulate system promised as a novel MRI nanoprobe for early diagnosis of prostate cancer （PCa）.

关 键 词：超顺磁性氧化铁 甲氧基聚乙二醇 接枝共聚物 纳米探针 前列腺癌 MRI 磁性纳米颗粒 单克隆抗体 

分 类 号：Q95-33[生物学—动物学] O623.413[理学—有机化学]