异丙酚对全身高温大鼠的脑保护作用  

Effect of propofol upon brain after whole-body hyperthermia in rats

作　　者：赵鑫[1] 金延武[1] 王端玉[2] 王志刚[2] 

机构地区：[1]山东大学第二医院麻醉科,济南250033 [2]山东大学齐鲁医院麻醉科

出　　处：《中华医学杂志》2009年第33期2356-2359,共4页National Medical Journal of China

基　　金：山东省科学技术发展计划项目（2007GG20002014）

摘　　要：目的探讨异丙酚对全身高温所致大鼠急性脑损伤的保护作用。方法60只健康雄性Wister大鼠随机分为6组（n=10）：水合氯醛麻醉组（B0组）、水合氯醛麻醉高温组（B组）、异丙酚100mg／kg麻醉组（C。组）、异丙酚100mg／kg麻醉高温组（C组）、异丙酚150mg／kg麻醉组（D0组）、异丙酚150mg／kg麻醉高温组（D组）。实验前所有大鼠在Morris水迷宫定位航行训练5d形成记忆，全身高温后24h再次进行定位航行试验观察记忆功能变化，后断头取脑，采用TUNEL法检测大鼠海马神经元凋亡，透射电镜观察神经元超微结构的变化。结果全身高温后，C和D组以及B0、C0和D0组大鼠逃逸潜伏期均低于B组（P〈0．05），B0、C0和D0组分别低于B、C和D组（P〈0．05）；B、C和D组凋亡神经元百分比均较B0、C0和D0组增高（P〈0．05），C组和D组凋亡神经元百分比较B组降低（P〈0．05），D组凋亡神经元百分比较C组减少（P〈0．05）；热疗组大鼠海马区神经元超微结构形态发生改变，C、D组损伤较B组轻，D组损伤较C组轻。结论异丙酚可减少全身高温所致的大鼠海马神经元凋亡，对高温引起的大鼠急性脑损伤有保护作用。Objective To investigate the effect of propofol upon acute cerebral insult during wholebody hyperthermia （WBH） in rats. Methods Sixty male Wister rats were randomly divided into 6 groups. All the animals were trained with place navigation test of Morris water maze （MWM） for 5 days pre-study. After training, some rats received anesthesia only but not WBH treatment as control, other rats were anesthetized with intraperitoneal injection of 3.0 ml/kg of chloral hydrate and 100 or 150 mg/kg of propofol followed with WBH process （keeping rat core temperature at 42℃ for 30 vain）. Space memory was checked using spatial probe test 24 h after WBH intervention. Rat brain was then removed in 24 h post-WBH to separate hippocampal regions for the detection of neuronal apoptosis with TUNEL method and ultramicrostructural changes by electron microscope. Results The escape latency were smaller in groups B0, C0, D0, C and D than that in group B （P〈0. 05） while smaller in groups B0, C0, D0 than in groups B, C, D respectively. The amount of apoptotic neurons in hippocampus increased orderly as following with no significant differences in group B0, CO and D0： group B0, C0, D0 〈 group D 〈 group C 〈 group B （P 〈 0.05 ）. The injury degree of neuronal ultramicrostructure in hippocampus in group C after WBH were more severe than that in group D but better than that in group B. Conclusion Propofol alleviates the WBHinduced acute cerebral insult by inhibiting the hippocampal neural apoptosis.

关 键 词：二异丙酚 高温 诱发 脑保护 

分 类 号：R686[医药卫生—骨科学]