不同年龄兔关节软骨细胞的力学特性  

Age-related biomechanical properties of cbondrocytes in rabbit knee articular cartilage

作　　者：卫小春[1] 李春江[1] 张全有[2] 陈维毅[2] 

机构地区：[1]山西医科大学第二医院骨科,太原030001 [2]太原理工大学应用力学研究所

出　　处：《中华医学杂志》2009年第33期2360-2363,共4页National Medical Journal of China

基　　金：国家自然科学基金（30872616）;山西省归国留学人员重点项目（200605）

摘　　要：目的探讨不同年龄关节软骨细胞的黏弹性特性。方法新西兰白兔分为幼年组、成年组和老年组。各组动物处死后，取双膝关节软骨并进行软骨细胞分离。采用微管吸吮技术结合半无限体细胞力学模型对关节软骨细胞进行黏弹性检测。结果不同年龄兔关节软骨细胞均表现为典型的黏弹性固体蠕变特性。在微管吸吮实验中，老年兔软骨细胞吸入长度随时间的变化与幼年及成年兔软骨细胞明显不同，表现在吸入长度较大、长度变化速度较快及达到平衡时间较短，而幼年及成年兔软骨细胞的变化趋势是相似的。随着年龄的增长，其黏弹性参数（瞬时模量民、平衡模量E∞、表观黏性μ）逐渐降低，幼年组E0为（0．67±0．10）kPa、E∞为（0．37±0．09）kPa、μ为（6．29±0．92）kPa·s；成年组‰为（0．65±0．07）kPa、E∞为（0．35±0．05）kPa、μ为（6．01±0．89）kPa·s；老年组民为（0．55±0．05）kPa、E∞为（0．28±0．04）kPa、μ为（4．10±0．61）kPa·s。分析表明老年组软骨细胞的黏弹性明显低于幼年组和成年组（P〈0．001），而后两组问差异无统计学意义（P〉0．05）。结论不同年龄的关节软骨细胞都表现出典型的黏弹性固体蠕变特征；老年关节软骨细胞的黏弹性特性较幼年和成年软骨细胞明显下降。Objective To fully demonstrate the alterations about the viscoelastic properties of chondrocytes in rabbit knee articular cartilage. Methods Fifteen New Zealand white rabbits were divided into 3 age groups： young group （ 1 month）, adult group （8 months） and old group （31 months）. All rabbits were sacrificed and isolated from knee joint and digested into chondrocytes. The micropipette aspiration combined with Half-space model was used to quantify changes in viscoelastic properties of chondrocytes. Results Experimental studies have shown that the changes of aspiration length with time in old group were obviously different from young group and adult group. But similar variations were found between the latter two groups. The viscoelastic properties of chondrocytes in old group exhibited a significantly lower instantaneous modulus E0 （ 0. 55 ± 0. 05 kPa）, equilibrium modulus E∞ （ 0. 28 ± 0. 04 kPa） and apparent viscosity （4. 10±0.61 kPa · s）as compared with young group （0. 67 ±0. 10）, （0.37 ± 0.09）, （6.29 ±0.92） kPa.s （P〈0.001） versus adult group：（0.65 ±0.07）,（0.35 ±0.05）,（6.01 ±0.89）kPa·s （P〈 0. 001 ）. But no difference were found between the latter two groups （ P 〉 0. 05 ）. Conclusion In response to a step pressure, chondrocytes in each group exhibited the viscoelastic solid creep behavior. The viscoelastic properties of ehondroeytes markedly decreased in old group.

关 键 词：软骨细胞 骨关节炎 微管 力学 

分 类 号：R686[医药卫生—骨科学]