促酰化蛋白通过PLC信号途径调控脂滴相关蛋白TIP47的表达  

Regulation of acylation stimulating protein on expression of lipid droplets associated protein TIP47 through PLC signal pathway

作　　者：梁晓燕[1] 卢慧玲[1] 胡秀芬[1] 吴静[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,湖北武汉430030

出　　处：《基础医学与临床》2009年第9期897-900,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30600213)

摘　　要：目的研究在3T3-L1脂肪细胞诱导分化过程中促酰化蛋白(ASP)对脂滴相关蛋白TIP47表达的影响,及阻断细胞PLC信号途径后此影响的变化。方法(1)对诱导分化过程中的3T3-L1脂肪细胞分别给予ASP、U73122及U73122和ASP处理,并设立相应空白对照;(2)RT-PCR检测细胞中TIP47mRNA的表达,Westernblot检测细胞中TIP47蛋白的表达。结果(1)ASP对3T3-L1脂肪细胞中TIP47mRNA和蛋白表达有显著的上调作用;(2)PLC信号途径阻断剂U73122对其有显著的下调作用;(3)U73122和ASP处理后脂肪细胞中TIP47mRNA和蛋白表达水平较仅ASP处理的脂肪细胞有显著降低,而较仅U73122处理的脂肪细胞有明显增高。结论PLC信号途径参与ASP调节TIP47表达的信号转导,从分子水平深化了对ASP成脂作用的认识,为肥胖症的认识及防治开拓新的思路。Objective To study the effect of acylation stimulating protein （ASP） on lipid droplets associated protein TIP47 during the differentiation process of adipocytes and the change of this effect after blocking PLC signal pathway. Methods （1）To treat 3T3-L1 adipocytes at indicated time point during differentiation with ASP, U73122 U73122 and ASP respectively, including control group in the same time. （2） To examine the mRNA and protein expression levels of TIP47 by RT-PCR and Western blot technique respectively. Results （ 1 ） Exposure to ASP for certain time resulted in an obvious increase of TIP47 gene and protein expression in 3T3-L1 adipocytes; （2）Expo- sure to U73122 which is a blocking agent of PLC signal pathway for certain time resulted in the decrease of TIP47 gene and protein expression in 3T3-L1 adipocytes; （3）Gene and protein level of TIP47 in adipocytes treated with both U73122 and ASP was significantly lower than that in adipocytes treated only with ASP, while was higher than that in adipocytes treated only with U73122. Conclusion PLC signal pathway plays a role in signal transduction of regulation of ASP on expression of TIP 4 7 . These results improve the understanding of adipogenesis of ASP on molecular level, and offer some new thinking in etiology, prevention and treatment of obesity.

关 键 词：促酰化蛋白 U73122 TIP47 3T3-L1脂肪细胞 肥胖症 

分 类 号：R589.2[医药卫生—内分泌]