热休克蛋白在涎腺肿瘤中的表达  

Expression of heat shock proteins in salivary tumor

作　　者：王桂兰[1] 顾晓林[2] 陈莉[1] 鄂群[1] 曹斌[3] 

机构地区：[1]南通大学医学院病理教研室,江苏南通226001 [2]南通大学医学院第二附属医院口腔科,江苏南通226001 [3]南通大学医学院附属海安人民医院病理科,江苏南通226000

出　　处：《基础医学与临床》2009年第9期948-954,共7页Basic and Clinical Medicine

基　　金：南通市指令性社会发展科技计划(S2006009)

摘　　要：目的研究热休克蛋白(HSPs)在涎腺良性、恶性肿瘤中的表达特征,探讨其对涎腺肿瘤发生发展的影响及意义。方法用免疫组化(S-P)法和免疫荧光双标记激光扫描共聚焦显微镜法检测41例良性和17例恶性肿瘤中HSPs的表达。采用stata7.0卡方检验和spearman相关分析。结果(1)5种HSPs在良、恶性肿瘤中均明显表达,且良性混合瘤的HSP84(β)和HSP86(α)的表达率均显著高于腺瘤和腺淋巴瘤(P<0.01)。(2)HSP27在恶性肿瘤中表达率显著下降,与肿瘤恶性显著负相关(r=-0.3506,P<0.01),同时HSP70、HSP86(90α)在恶性肿瘤中的表达显著增加,尤其HSP70/HSP86(90α)在恶性肿瘤中双阳性表达率高达100%(Fisher s exactP<0.05)。结论(1)HSPs共同参与了涎腺肿瘤的发生,但存在表达种类和率的差异。(2)HSP27表达减弱可能是涎腺肿瘤分化降低的标志。(3)HSP70、HSP90,尤其HSP86(90α)可能通过致瘤顾客蛋白的持续活化促使恶性肿瘤发生发展,以HSP86(90α)为主的Hsp90可能是涎腺恶性肿瘤治疗的靶点。Objective The expression characteristics of heat shock proteins （HSPs） in salivary tumor were studied to find their effect and relationship on the occurrence and development of salivary tumor. Methods With immunohistochemistry techniques S-P, Immunofluorescence Double Staining Technique and Confocal Laser Scanning Microscope（CLSM） ,expression of HSPs was checked in 41 cases of benign tumors and 17 malignant tumors. The data were analyzed with stataT. 0 chi square test and spearman correlation analysis. Results （ 1 ） All 5 HSPs were significantly expressed both in benign and malignant tumors. The expression rate of HSP84 （13） and HSP86 （α）was higher in benign mixed tumor （BMT）than in adenoma or adenolymphoma （P 〈 0. 01 ）. （2）In malignant tumors, the expression of HSP27 was significantly lower（ r = - 0. 3506, P 〈 0.01 ）, negatively related to malignant, while the expression of HSP70 or HSP86 （ 90α ） increased , especially the double expression ratio of HSP70/HSP86 （90α） reached 100%. Conclusion （1）All 5 HSPs might be involved in tumorigenesis,but the types and expression rate were a little different. （2） The lower-expression of HSP27 might be the biomarker of differentiation depression in salivary tumor. （3）HSP70 and HSP90,especially HSP86（90α） may promote occurrence and development of malignant tumors through the persistence-activation of tumorigenesis client proteins. HSP90, especially HSP86 （90α） ,may be a target of salivary oncotherapy.

关 键 词：涎腺肿瘤 热休克蛋白 免疫组化 免疫荧光双标记 激光扫描共聚焦显微镜 

分 类 号：R739.8[医药卫生—肿瘤]