斑马鱼和鲢对微囊藻毒素与孔雀石绿的行为反应  

Behavior Response of Zebrafish,Danio rerio and Silver carp,Hypophthalmichthys molitrix to Microcystins and Malachite Green

作　　者：程炜轩[1] 梁旭方[1] 王琳[1] 林小涛[1] 

机构地区：[1]暨南大学生命科学技术学院,广东广州510632

出　　处：《水生态学杂志》2009年第5期36-40,共5页Journal of Hydroecology

基　　金：国家科技部863项目(2007AA09Z437);国家自然科学基金项目(30670367);广东省科技计划项目(2007B020701002;2005B20301005);广东省自然科学基金项目(031886)

摘　　要：鱼类行为能准确地反映环境变化,并且随着污染物浓度的变化而发生改变,因此将毒素敏感鱼类的行为作为标记,用以监测环境是可行的。用人工饲养、对环境毒素敏感的斑马鱼(Daniorerio)和对环境毒素有较强吸收能力的鲢(Hypophtha lmichthys molitrix)作为指示生物,检测其行为对水体中微囊藻毒素(MC)和孔雀石绿(MG)的反应。结果表明,斑马鱼和鲢在2μg/LMC浸泡下未出现显著的行为反应,而在5μg/L的MC浸泡下,斑马鱼摆尾频率在10min后、移动速度在40min后显著性下降;鲢的摆尾频率与移动速度均在40min后显著性下降;0.5mg/LMG处理下,斑马鱼未出现显著性行为变化,而鲢摆尾频率与移动速度均在35min后显著性下降。Behavior index of fish accurately reflected the alteration of environment. In addition, it could monitor the existence of contamination and followed the concentration change of contamination, so it was feasible to use the sensitive fish to monitor the environment. This study used Danio rerio,which was a sensitive fish to environmental toxin and Hypophthalmichthys molitrix, which had strong absorbability to environmental toxin to be the eucoen, and behavior index of these two fishes was used to monitor the existence of microcystins （ MC ） and malachite green （MG）. The results showed that, when exposed to 2 μg/L MC-LR, no significant behavior response of Danio rerio and Hypophthalmichthys molitrix were observed. When exposed to 5 μg/L MC-LR, tail beat frequency of Danio rerio and Hypophthalmichthys molitrix decreased significantly after 10 min and 40 min, respectively. Swimming velocity of Danio rerio and Hypophthalmichthys molitrix both decreased significantly after 40 min. When exposed to 0.5 mg/L MG, no significant behavior response of Danio rerio was observed, whereas tail beat frequency and swimming velocity of Hypophthalmichthys molitrix decreased significantly after 35 min.

关 键 词：微囊藻毒素 孔雀石绿 斑马鱼 鲢 行为反应 

分 类 号：Q503[生物学—生物化学]