皂土对RNA的有效分离和保护作用的初步研究  

A primary study on efficient isolation and protection of RNA by bentonite

作　　者：郑彦峰[1] 淳俊[1] 李亮[1] 李淳[1] 张荣[1] 王胜华[1] 陈放[1] 

机构地区：[1]生物资源与生态环境教育部重点实验室四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2009年第5期1558-1562,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家重点研究发展技术项目(2007BAD50B01)

摘　　要：以富含多糖多酚的麻疯树,富含RNase的动物肝脏,以及霉菌为实验材料,利用皂土-SDS法分别提取其总RNA,A_(260)/A_(280均)在1.8以上,结果表明皂土-SDS法是一种广泛适用的RNA提取法.此外,以皂土-DEPC水为抑制剂,分析其抑制RNase的作用,实验表明皂土与常用抑制剂Rnasin的效果相当.同时,实验证明皂土作为RNase抑制剂对RT-PCR等分子生物学后续实验的并无影响.Bentonite-SDS method is introduced. The total RNAs were successfully extracted from RNaserich rabbit liver and plant,microbial tissues rich in polysaccharides and polyphenol compounds with the ratios of A260/A280〉 1.8. Besides, the anti-RNase activities of bentonite have been tested. The results showed that bentonite posses the same inhibitive effects against RNase as Rnasin for the tolerance of high temperature. The RNA protected by bentonite was confirmed to be used for following molecular biological research by RT-PCR. The above described indicates that bentonite can be applied as RNase inhibitor.

关 键 词：RNA提取 皂土-SDS法 皂土 RNase抑制剂 

分 类 号：Q781[生物学—分子生物学]