Rose bengal处理对植物类囊体膜和PSⅡ颗粒活性的影响  

Effects of Rose bengal on activity of thylakoid membrane and PSⅡ particle

作　　者：袁瀚[1] 康庄[1] 胡源[1] 蒋佳宏[1] 杜林方[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2009年第5期1563-1568,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(30270124;39970068)

摘　　要：研究了Rose bengal处理对菠菜类囊体膜及PSⅡ颗粒的叶绿素荧光发射光谱、蛋白质内源荧光发射光谱及DCIP光还原活性的影响.结果表明:单线态氧可改变类囊体膜的结构,并且可破坏PSⅡ反应中心及LHCⅡ中叶绿素分子的结合状态,引起类囊体膜PSⅡ天线系统中的叶绿素捕光效率下降,还可引起光合电子传递能力下降和类囊体膜蛋白构象的改变.Treat spinach thylakoid membrane and PS Ⅱ particle with rose bengal （RB） in different concentration, which can generate 1O2 via illuminate by green light. Then the effects of Oz on fluoresence emission spectra at room temperature, activity of DCIP photoreduction were studied. The results showed that 1O2 can change the protein conformation in thylakoid membrane, destroy PS Ⅱ reaction centre and the combine of chlorophyll in light harvest complex Ⅱ （LHC Ⅱ ）, so, reduce the light harvesting efficiency of LHC Ⅱ. This research also concluded that 1O2 can reduce the efficiency of electron transfer chain in thylakoid membrane.

关 键 词：ROSE BENGAL 单线态氧 类囊体膜 PSⅡ颗粒 

分 类 号：Q946[生物学—植物学]