刺芒柄花素对大鼠血脂及肝脏雌激素受体的作用  

Effect of formononetin on serum lipids and hepatic estrogen receptors

作　　者：幸奠霞[1] 薛存宽[1] 黄畦[1] 刘志高[1] 鲁敏[1] 曾伶[1] 何学斌[1] 

机构地区：[1]华中科技大学同济医学院附属梨园医院,湖北武汉430077

出　　处：《中国医院药学杂志》2009年第18期1558-1561,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的:研究刺芒柄花素对大鼠血脂的调节作用和对肝脏雌激素受体表达的影响。方法:雌性SD成年大鼠40只,随机分为5组,即假手术组、模型组、尼尔雌醇组(阳性对照)、刺芒柄花素低剂量组、刺芒柄花素高剂量组,每组8只。假手术组打开腹腔后切除卵巢周围小块脂肪后关腹,后4组行双侧卵巢切除术,术后第5～10天每天1次进行阴道冲洗脱落细胞涂片,以了解去势是否彻底。确认去势成功后各组饲以高脂饮食,同时,假手术组和模型组灌胃生理盐水1mL.(100g)-1.d-1,尼尔雌醇组灌胃尼尔雌醇每周2.5mg·kg-1,刺芒柄花素低剂量组灌胃刺芒柄花素20mg·kg-1.d-1,刺芒柄花素高剂量组灌胃刺芒柄花素100mg·kg-1.d-1,连续8周。第8周后5d连续作阴道冲洗细胞涂片,第8周末断头处死大鼠,取血测血脂,取肝脏组织进行逆转录聚合本科链反应(RT-PCR),测定ERα、ERβ的相对表达量。结果:尼尔雌醇组和高剂量刺芒柄花素组血清TC、LDL-C明显降低,TG和HDL-C无明显改变;高剂量刺芒柄花素选择性上调去势大鼠肝脏ERβ表达。结论:刺芒柄花素能有效改善去势后高脂饮食大鼠血脂紊乱,这一作用可能是通过ERβ实现的。OBJECTIVE To observe the effect of formononetin on serum lipids and the expression of hepatic estrogen recep tors. METHODS 40 femal rats were divided into five groups randomly： the sham-operated group, model group, nilestriol group, formononetin groups of low and high dose. A piece of fat of rats in sham-operated group was cut before closing the abdomen,the others were ovarieetomized. To test whether the model was successfully or not, vaginal exfoliated cell were observed from the fifth day to the tenth after operation. Rats were fed with after anmimal models were confirmed to be successful. In the mean time, The sham-operated and model group were given with nomal saline solution in 1 mE. （100 g）^- 1·d^-1 by gavage, the remaining three groups were given nilestriol 2. 5 mg·kg^-1 a week,low（20 mg·kg^-1·d^-1）and high dose（100 mg·kg^-1·d^-1）of formononetin by gavage respectively. In the 8th week, vaginal exfoliated cell were observed, then decapitated the rats, collected blood to detect the serum lipids level,removed the hepatic tissue to take RT-PCR, detected the relative expression of ERα and ERβ. RESULTS High dose of formononetin（100 mg·kg^-1 ） decreased the level of TC and LDL C, and Selectively up-regulated expression of ERβ. CONCLUSION Formononetin ameliorate lipids disorders of ovariectomized rats fed high-fat diet, and it may be achieved through the ERβ.

关 键 词：刺芒柄花素 大鼠 血脂 雌激素受体 

分 类 号：R69[医药卫生—泌尿科学]