化瘀理肺方对模型大鼠肺纤维化的保护作用研究  

Protective Effect of Huayu Lifei Prescription on Model Rats against Pulmonary Fibrosis

作　　者：毕建伟[1] 刘世青[2] 周玲[2] 史广超[1] 王琴[1] 鹿伟[3] 

机构地区：[1]山东大学医学院,济南市250012 [2]山东大学附属千佛山医院呼吸科,济南市250014 [3]山东大学附属千佛山医院病理科,济南市250014

出　　处：《中国药房》2009年第27期2092-2094,共3页China Pharmacy

基　　金：山东省科技发展计划项目(2006GG2302030)

摘　　要：目的:研究化瘀理肺方对肺纤维化模型大鼠肺纤维化的保护作用。方法:将大鼠随机分为4组,即正常对照、模型、化瘀理肺方、激素组。通过气管内插管注入博来霉素(5mg·kg-1)复制肺纤维化模型。复制模型后第2天,化瘀理肺方组大鼠灌胃化瘀理肺方颗粒(17190mg·kg-1),激素组大鼠腹腔注射氢化可的松琥珀酸钠(25mg·kg-1),正常对照和模型组大鼠灌胃0.25mL生理盐水。在给药后的第7、14、28天每组分别处死7只,分离、称取肺组织,计算肺系数,取右肺组织匀浆,采用双抗体酶联免疫吸附法测定匀浆液中肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白介素-4(IL-4)的含量。结果:化瘀理肺方能显著减轻肺泡炎症和肺纤维化的程度;与模型组比较,化瘀理肺方组IFN-γ显著升高(P<0.01),IL-4、TNF-α显著降低(P<0.01)。结论:化瘀理肺方有一定抗肺纤维化的作用,其机制与调节辅助性T淋巴细胞Th1/Th2细胞之间的平衡及抑制单核巨噬细胞的活性有关。OBJECTIVE：To explore the protective effect of Huayu lifei prescription on pulmonary fibrosis model rats against pulmonary fibrosis.METHODS：The rats were randomly divided into 4 groups：normal control group, model group, Huayu lifei prescription group, and hydrocortisone group. The pulmonary fibrosis model was induced by bleomycin（5mg·kg^-1）. On the second day after modeling, the Huayu lifei prescription group received Huayu lifei prescription granula（17190mg·kg^-1） ig. ; the hydrocortisone group received Hydroeortisone sodium succinate（25mg·kg^-1） by intraperitoneal injection, while the normal control group and the model group received 0.25 mL normal saline .Seven rats in each group were killed on 7,14,28 days after drug intervention, with the lung tissue separated and weighed and the lung indexes calculated. The right lung tissue homogenate was taken for determination of levels of TNF-α, IFN-γ and IL -4 using double antibody enzyme linked immunosorbent assay. RESULTS： Huayu lifei prescription significantly alleviated alveolitis and pulmonary fibrosis in rats. As compared with model group, the rats in Huayu lifei prescription-treated group showed significantly increased IFN-y content but significant reduction in IL- 4 content and TNF-α content（P 〈 0.01） . CONCLUSION： Huayu lifei prescription can alleviate the degree of pulmonary fibrosis, which might be associated with its regulatory effect on the balance between Th1 and Th2 cells and the inhibitory effect on the activity of mononuclear macrophages.

关 键 词：肺纤维化 化瘀理肺方 肿瘤坏死因子-Α 干扰素-Γ 白介素-4 

分 类 号：R285[医药卫生—中药学] R97[医药卫生—中医学]