HPLC-ELSD法测定丹芪偏瘫胶囊中黄芪甲苷的含量  

Determination of Astragaloside Ⅳ in Danqi Piantan Capsule by HPLC-ELSD

作　　者：刘雪平[1] 徐跃红[2] 

机构地区：[1]天津中医药大学第一附属医院制剂室,天津市300193 [2]天津中医药大学中药学院,天津市300193

出　　处：《中国药房》2009年第27期2135-2137,共3页China Pharmacy

摘　　要：目的:建立以高效液相色谱-蒸发光散射法测定丹芪偏瘫胶囊中黄芪甲苷含量的方法。方法:色谱柱为Intertex C18(250mm×4.6mm,5μm),流动相为乙腈-水(32∶68),流速为1mL.min-1,柱温为室温。结果:黄芪甲苷检测浓度在0.0915～0.915mg·mL-1范围内的对数值与峰面积积分值的对数值呈良好的线性关系(r=0.9995);平均回收率为101.12%,RSD=1.75%(n=6)。结论:本方法操作简便、结果准确、重现性好,可用于丹芪偏瘫胶囊的质量控制。OBJECTIVE： To establish an HPLC-ELSD method for the determination of the content of astragaloside Ⅳ in Danqi piantan capsule.METHODS： The determination was performed on Intertex C18 column（250mm×4.6mm,5μm） with column temperature kept at room temperature. The mobile phase consisted of acetonitrile - water（32 ： 68） at a flow rate of 1 mL·min^-1.RESULTS： Astragaloside IV showed good linear relationship in the range of 0.091 5-0.915 mg·mL^-1（r = 0.999 5）, and the average recovery of astragaloside Ⅳ was 101.12% （RSD = 1.75%, n = 6）. CONCLUSION ： The method is simple, accurate and reproducible, and it is applicable for the quality control of Danqi piantan capsule.

关 键 词：丹芪偏瘫胶囊 高效液相色谱-蒸发光散射法 黄芪甲苷 含量测定 

分 类 号：R283.65[医药卫生—中药学] R927.2[医药卫生—中医学]