北京市交警多环芳烃暴露及DNA氧化损伤水平研究  

Polycyclic Aromatic Hydrocarbons Exposure and DNA Oxidative Damage in Traffic Policemen of Beijing

作　　者：王秦[1] 徐春雨[1] 徐东群[1] 黄宁华[2] 汪静[3] 常君瑞[1] 董小艳[1] 李韵谱[1] 李红[1] 方建龙[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021 [2]北京大学医学部公共卫生学院,北京100191 [3]北京市崇文区疾病预防控制中心,北京100050

出　　处：《环境与健康杂志》2009年第9期770-773,共4页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(40575064)

摘　　要：目的了解北京市交警多环芳烃(PAHs)暴露对体内DNA氧化损伤水平的影响。方法于2007年6-8月,以某城区41名男性外勤交警和34名男性郊区对照人群作为研究对象,进行问卷调查及环境空气PAHs浓度、内暴露指标尿中1-羟基芘(1-OHP)和DNA氧化损伤标志物尿中8-羟基脱氧鸟苷(8-OHdG)的检测,通过单因素及多元回归分析PAHs内外暴露水平对8-OHdG的影响。结果交警组工作环境空气中细颗粒物(PM2.5)、苯并(a)芘[B(a)P]、PAHs浓度分别为0.096mg/m3、3.20ng/m3、38.32ng/m3,高于郊区居民组相应值0.045mg/m3、1.54ng/m3、25.43ng/m3;交警组和郊区居民组人群尿中1-OHP浓度分别为(0.50±0.38)和(0.34±0.28)μmol/mol Cr(P<0.05),尿中8-OHdG浓度分别为(13.74±6.30)和(11.61±6.02)ng/mg C(rP>0.05)。多因素分析发现尿中1-OHP和吸烟是影响8-OHdG水平的重要因素,没有发现年龄、饮酒、锻炼习惯等调查因素对尿8-OHdG浓度的影响。结论北京市交通污染对交警这一职业人群已产生一定的健康影响,环境空气PAHs暴露和吸烟是影响研究人群DNA氧化损伤水平的重要因素。Objective To explore polycyclic aromatic hydrocarbons （PAHs） exposure of traffic policemen in Beijing and its impact on DNA oxidative damage. Methods From Jan. to Aug. 2007, 41 male traffic policemen and 34 male suburban inhabitants in Beijing were selected. The ambient fine particulate matter （PM2.5） was sampled within two consecutive days in the work places of traffic policeman and in the residential area of suburban inhabitants respectively. The levels of urinary 1-hydroxypyrene （1-OHP） and 10 PAHs species were both analyzed by high performance liquid chromatography （HPLC）. The levels of urinary 8-hydroxy-2＇-deoxyguanosine （8-OHdG） were detected by ELISA kit, while the questionnaires for health information on smoking, drinking alcohol, exercise habit and so on were also inquired. Results The average levels of PM2.5, benzo[a]pyrene [B （a）P] and total PAHs in the traffic policeman group were 0.096 mg/m3, 3.20 ng/m3, and 38.32 ng/m3 respectively, while the corresponding values in the suburban inhabitant group were 0.045 mg/m3, 1.54 ng/m3 and 25.43 ng/m3 respectively. The concentration of urinary 1-OHP in the policeman group [（0.50±0.38 ）μmol/mol Cr] was higher than that of the suburban inhabitant group [（0.34±0.28） μmol/mol Cr], P〈0.05. The average level of 8-OHdG in policemen was （13.74±6.30）ng/mg Cr, while that of suburban inhabitants was （ 11.61±6.02 ）ng/mg Cr, P〉0.05. Multiple factors analysis indicated that urinary 1-OHP and smoking habit had influence on urinary 8-OHdG level, while there was no relationship with other factors （such as drinking alcohol, exercise and cooking habit）. Conclusion Traffic pollution may increase the level of DNA oxidative damage in policemen in Beijing.Traffic and smoking habit-related PAHs exposure is the important influencing factor.

关 键 词：空气污染 交警 郊区居民 多环芳烃 8-羟基脱氧鸟苷 1-羟基芘 

分 类 号：R122.2[医药卫生—环境卫生学] R181.3[医药卫生—公共卫生与预防医学]