淫羊藿黄酮对阿尔茨海默病小鼠模型学习记忆能力的影响  

Effects of Epimedium flavanoids on learning and memory ability in Alzheimer＇ s disease mouse model

作　　者：叶翠飞[1] 宋秋洁[2] 艾厚喜[1] 李林[1] 

机构地区：[1]首都医科大学宣武医院药物研究室,神经变性病教育部重点实验室,北京100053 [2]国家食品药品监督管理局药品评价中心

出　　处：《中华行为医学与脑科学杂志》2009年第9期769-771,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：国家自然科学基金项目（90709011,30472184）;国家重点基础研究发展计划“973”计划项目（2003CB517104）

摘　　要：目的观察淫羊藿黄酮对β-淀粉样肽（Aβ）拟阿尔茨海默病（AD）模型小鼠学习记忆能力的影响。方法将ICR小鼠随机分为正常组、假手术组、模型组，淫羊藿黄酮小剂量（0．03g／kg）、中剂量（0．1g／kg）、大剂量（0．3g／kg）组。灌胃给药14d后，行右侧脑室注射Aβ1-40造模，继续给药7d，进行Morris水迷宫、避暗实验和自主活动实验，观察小鼠学习记忆能力。结果Aβ1-40侧脑室注射模型小鼠出现学习记忆障碍。淫羊藿黄酮中、大剂量组在Morris水迷宫实验中逃避潜伏期[（40．12±4．15）S，（34．99±5．49）S]和游泳距离[（648．36±88．42）cm，（781．57±104．41）cm]明显缩短，与模型组逃避潜伏期（65．45±5．15）S，游泳距离（1142．66±96．80）cm比较，差异具有显著性（P〈0．01），在避暗实验中可延长模型小鼠的潜伏期[（255．40±11．00）S，（257．46±19．50）S]并降低其错误次数[（0．80±0．14）次，（0．77±0．17）次]，与模型组潜伏期[（196．27±25．47）S]，错误次数[（1．47±0．31）次]比较，差异有显著性（P〈0．05）。在自主活动实验中，各组之间差异无显著性（P〉0．05）。结论淫羊藿黄酮能明显提高Aβ脑室注射致AD小鼠模型的学习记忆能力。Objective To investigate the effects of Epimedium flavanoids（EF） on learning and memory ability in Alzheimer＇ s disease （AD） mouse model induced by β-amyloid （Aβ） lateral ventricle injection. Meth- ods ICR mice were divided into normal group, sham operation group,model group,positive control drug （Ibupro- fen） group,EF low dose （0.03 g/kg） ,middle dose （0.1 g/kg） and high dose （0.3 g/kg） groups. The model was induced by injecting Aβ1-40 into right lateral ventricle of mice. In the treatment groups,EF was introgastrically ad- ministrated 14 d before injection and 7 d after injection. The learning and memory ability was determined by Morris water maze, step through and spontaneous locomotor activity tests. Results The function of learning-memory was significantly decreased in mouse model induced by Aβ lateral ventricle injection. Administration of middle and high dose EF significantly decreased the escape latency[ （40.12 ± 4.15 ）s, （34.99 ± 5.49）s] and swimming dis- tance [（648.36±88.42）em,（781.57±104.41）cm] than that of the model group（65.45 ±5. 15）s,（1142.66 ± 96.80） cm, P 〈 0.01 ） in Morris water maze test, and prolonged the latent period [ （ 255.40 ± 11.00 ） s, （ 257.46 ± 19.50 ） s ] and decreased the error times （ O. 80 ± 0.14,0.77 ± 0.17 ） in step-through test significantly, compared with the model mice [ （ 196.27 ± 25.47 ） s, （ 1.47 ± 0.31 ） ] （ P 〈 0.05 ）. In the spontaneous locomotor activity ex- periment,no obvious difference was found among all groups（P〉 0.05 ）. Conclusion EF significantly improved the learning and memory ability in AD mouse model induced by Aβ1-40 lateral ventricle injection.

关 键 词：淫羊藿黄酮 Β-淀粉样肽 阿尔茨海默病 学习记忆 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]