脾虚大鼠中枢单胺类神经递质含量及四君子汤干预的研究  

The research of spleen deficiency rats＇ monoamine neurotransmitter and the treatment mechanism of Si- junzi Decoction

作　　者：黄小波[1] 李宗信[1] 陈文强[1] 王宁群[1] 周茗[2] 吴燕川[3] 郭德玉[4] 

机构地区：[1]首都医科大学宣武医院中医科,北京100053 [2]首都医科大学宣武医院神经内科实验室 ,北京100053 [3]首都医科大学宣武医院中心实验室 ,北京100053 [4]首都医科大学宣武医院动物实验室 ,北京100053

出　　处：《中华行为医学与脑科学杂志》2009年第9期779-780,共2页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：国家自然科学基金资助项目（30772843）;北京市自然科学基金资助项目（7053068）;北京市中医管理局资助北京市中西医结合老年神经病学重点学科建设项目（京中重V126）

摘　　要：目的研究脾虚大鼠中枢单胺类神经递质含量的变化和四君子汤干预的途径。方法将60只大鼠分为空白对照组、脾虚组、四君子汤组，每组各20只，使用高效液相检测大鼠纹状体中5-HT、DA、5-HIAA、DOPAC和HVA的含量。结果与对照组比较，脾虚组大鼠纹状体内5-HT（2．25±0．78）ng／mg水平显著升高，DA（26．06±9．01）ng／mg水平显著下降（P〈0．01）；脾虚大鼠使用四君子汤干预后，纹状体内5-HT（1．86±0．53）ng／mg和DA（38．95±10．86）ng／mg水平基本恢复正常，与对照组差异无显著性，而DA水平与脾虚组大鼠差异有显著性（P〈0．05）。结论脾虚证大鼠纹状体中的单胺类神经递质的含量异常，脾虚证存在有中枢机制，健脾名方四君子汤可以调节脾虚大鼠中枢单胺类神经递质含量。Objective To analyze the change of spleen deficiency rats＇ monoamine neurotransmitter and the treatment mechanism of Sijunzi decoction. Methods 60 rats were divided into control group, spleen deficiency group and Sijunzi Decoction group. And every group had 20 rats. Then high performance liquid chromatograph was used to analyze rats＇ 5-HT, DA,5-HIAA, DOPAC and HVA,in their corpus striatum. Results Compared with control group,the concentration of 5-HT[ （2.25 ± 0.78 ） ng/mg ] in spleen deficiency rats significantly ascended, and DA [ （ 26.06 ± 9.01 ） ng/mg] significantly descended （P 〈 0.01 ）. After the treatment of Sijuuzi decoction, the level of 5-HT [ （ 1.86 ± 0.53 ） ng/mg ] and DA [ （ 38.95 ± 10.86 ） ng/mg ] came back to normal. And compared with spleen deficiency rats,treatment rats＇ DA significantly ascended （P 〈 0.05）. Conclusion There is a con- sanguineous relationship between spleen deficiency syndrome and rats＇ monoamine neurotransmitter, and spleen deficiency syndrome has a central mechanism. And Sijunzi decoction could be used to treat with spleen deficiency syndrome by regulating monoamine neurotransmitter.

关 键 词：脾虚 单胺类神经递质 四君子汤 

分 类 号：R285.5[医药卫生—中药学] R277.722[医药卫生—中医学]