卷烟烟气有害成分联合作用的细胞毒性  

Synergic Cytotoxicity of Harmful Components in Cigarette Smoke

作　　者：刘荣[1] 潘秀颉[2] 杨陟华[2] 刘兴余[3] 张洋[1] 刘惠民[3] 齐绍武[1] 谢剑平[3] 朱茂祥[2] 

机构地区：[1]湖南农业大学农学院 [2]军事医学科学院放射与辐射医学研究所,北京市海淀区太平路27号100850 [3]中国烟草总公司郑州烟草研究院

出　　处：《烟草科技》2009年第9期27-31,共5页Tobacco Science & Technology

基　　金：国家烟草专卖局重点科技攻关项目"卷烟烟气主要有害成分生物学效应矢量法分析"(国烟科[2006]371号)

摘　　要：为评价卷烟烟气有害成分的细胞毒性及其联合作用,以人支气管上皮细胞(BEASe-2B细胞)为靶细胞,分别用卷烟烟气中5种有害成分4-甲基亚硝基-1-(3-吡啶基)-1-丁酮(NNK)、氨、苯并[a]芘、苯酚和巴豆醛单独及两两联合染毒,用MTT法测定染毒24h后的细胞存活率,计算细胞的平均半数致死剂量(LC50),用效应图解法对联合作用进行评价。结果显示:①NNK、氨、苯并[a]芘、苯酚和巴豆醛的LC50分别为87.2、395.5、5.4、61.9和6.9mg/L;②NNK与氨、NNK与苯并[a]芘、NNK与苯酚、NNK与巴豆醛、氨与苯并[a]芘、氨与苯酚、氨与巴豆醛、苯并[a]芘与苯酚、苯并[a]芘与巴豆醛、苯酚与巴豆醛的LC50分别为46.2、10.9、14.1、30.4、76.4、86.1、39.0、6.1、1.1和22.1mg/L;③NNK与氨、NNK与苯并[a]芘、NNK与苯酚、苯并[a]芘与苯酚、苯并[a]芘与巴豆醛、氨与巴豆醛存在明显的协同作用,而NNK与巴豆醛、苯并[a]芘与氨、苯酚与氨、苯酚与巴豆醛之间无协同作用。因此,卷烟烟气有害成分的细胞毒性存在明显差异,其联合作用的细胞毒性效应各不相同。In order to assess the individual and synergic cytotoxicities of harmful components in cigarette smoke, bronchus epithelia, BEASe - 2B, were used as target cells and exposed to the five harmful components in cigarette smoke, NNK, NH3, B[ a~ P, phenol and crotonaldehyde, individually and in pairs, the survival rates of the cells after 24 - hour exposure were tested by MTT assay, average LCso of the cells was calculated, and the synergic cytotoxicities were evaluated with isobolograms. The results showed that ： 1 ） LCso ofNNK, NH3, B[a]P, phenol, crotonaldehyde, NNK ＋NH3, NNK ＋B[a]P, NNK ＋ crotonaldehyde, NH3 ＋ B[ a] P, NH3 ＋ phenol, NH3 ＋ crotonaldehyde, B [ a]P ＋ phenol, B dehyde and phenol ＋ crotonaldehyde was 87.2, 395.5, 5.4, 61.9, 6.9, 46.2, 10.9, 14. enol, NNK ＋ ] P ＋ crotonal- 30.4, 76.4, 86. 1, 39.0, 6.1, 1.1 and 22.1 mg/L, respectively. It suggested that there existed potent synergism between NNK and NH3, NNK and B[ alP, NNK and phenol, B[ a]P and phenol, B[ a] P and crotonaldehyde, NH3 and crotonaldehyde, while no synergism between NNK and crotonaldehyde, B [ a]P and NH3, phenol and NH3, phenol and crotonaldehyde in cytotoxicity.

关 键 词：卷烟烟气 NNK 氨 苯并[A]芘 苯酚 巴豆醛 细胞毒性 

分 类 号：TS416[农业科学—烟草工业]