白黎芦醇与DNA相互作用研究  

Research on the Interaction of Resveratrol and DNA

作　　者：刘炜[1] 舒火明[1] 纪明慧[1] 谢雄仕 陈光英[1] 

机构地区：[1]海南师范大学化学与化工学院海南省热带药用植物化学重点实验室,海南海口571158

出　　处：《安徽农业科学》2009年第28期13441-13442,共2页Journal of Anhui Agricultural Sciences

基　　金：海南省重点科技项目(070207);海南师范大学博士启动基金资助项目(103307);海南省教育厅项目(Hjkj2009-39);海南省有机化学重点学科开放基金

摘　　要：[目的]为进一步研究白黎芦醇药理活性提供信息基础。[方法]在pH值为6的Tris缓冲溶液体系中，应用荧光光谱法和紫外光谱技术以及黏度法研究白黎芦醇和DNA分子间的相互作用，计算白黎芦醇与DNA的结合常数和结合位点数。[结果]25℃时，白黎芦醇与DNA之间的结合常数为3．96×10^3L／mol，结合位点数为1．0053，结合方式是白黎芦醇通过嵌入结合的方式与DNA结合，这种结合方式可能是白黎芦醇具有抗癌功效的一个重要因素，即白黎芦醇通过嵌入方式与DNA结合，影响DNA的转录和复制，从而导致癌细胞凋亡。[结论]证明白黎芦醇是以嵌入结合的方式与DNA结合，这种结合方式可能是白黎芦醇具有抗癌活性的一个重要原因。[ Objective ] The basis of the information for the further study on the pharmacological activity of resveratrol was provided. [ MethYl] The interaction between Resveratrol and DNA moleeulein in the Tris buffer solution system with the pH value of 6 was studied with the fluorescence spectroscopy, UV technology and the viscosity method. The binding constant and binding site between the resveratrol and DNA were calculated. [ Results] At 25℃, the binding constant and binding site between the resveratrol and DNA were 3.96 × 10^3 L/mol and 1. 005 3, respectively. The binding way was the resveratrol embedded into DNA, which could be an important factor that the resveratrol may have anti-cancer efficacy and the resveratrol embedded into DNA affected DNA transcription and replication, resulting in apoptosis of cancer cells. [ Conclusion ] It was proved that the resveratrol was embedded into DNA, which could be an important reason that the resveratrol had the anti-tumor activity.

关 键 词：白黎芦醇 DNA 嵌入结合 抗癌 

分 类 号：O657.3[理学—分析化学]