滇西二高温热泉原核生物多样性的比较分析  

Comparative Analysis of the Prokaryote Diversities of Two Hyperthermal Springs in the West of Yunnan

作　　者：李华兰[1] 王涛[1,2] 李沁元[3] 张东华[2] 彭谦[2] 崔晓龙[2] 

机构地区：[1]宜宾学院生命科学与食品工程系,四川宜宾644007 [2]云南大学云南省微生物研究所,云南昆明650091 [3]云南大学生命科学教学实验中心,云南昆明650091

出　　处：《安徽农业科学》2009年第28期13480-13481,13531,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目资助(30860013;30660004);云南省自然科学基金(2006C0006M);云南省中青年学术带头人后备人才基金(2005PY01-1)

摘　　要：直接提取腾冲大滚锅热泉壁、龙陵大沸泉泉底沉积物总DNA,PCR扩增获得古菌和细菌16S rDNA的V_8高变区片段,进行DGGE分析。结果显示,细菌和古菌在2个高温热泉中均有一定数量的存在,而且2个热泉古菌的DGGE条带差异度较大,而细菌在2个高温热泉中数量较多,而且相似较大。The gross DNA of two environmental samples was extracted from a hot spring Dagunguo of Tengchong Rehai and a hot spring Dafeiquan of Longling. Then, the fragments of Vs region of the 16S rRNA genes of bacteria and archaea were obtained by PCR using two sets of domain-specific primers, and these fragments were analyzed by DGGE （denature gradient gel electrophoresis）, respectively. The results of the DGGE analyses indicated that the two neighbor hot springs possesses relatively plenty of prokaryotic species with relatively high comparability between bacterial microfloraznd relatively low comparability between arcbaeal microflora.

关 键 词：高温热泉 DGGE 细菌 古菌 差异性 

分 类 号：S188[农业科学—农业基础科学]