慢性应激对小鼠NPY表达及学习记忆的影响  

Expression of Chronic Stress on NPY in Mice and the Impact of Learning and Memory

作　　者：马颖[1] 卢莉[2] 袁洁[2] 

机构地区：[1]山西医科大学第一医院神经外科 [2]山西医科大学医学心理学教研室

出　　处：《中国健康心理学杂志》2009年第9期1126-1128,共3页China Journal of Health Psychology

基　　金：山西省自然科学基金资助项目(051089);项目名称:复方疏肝胶囊对心理应激亚健康状态免疫调节的研究

摘　　要：目的通过观察慢性应激对小鼠学习记忆能力以及脑组织神经肽Y(NPY)含量的变化,探讨慢性应激对学习记忆和神经肽Y的影响。方法采用束缚制动的方法建立慢性心理应激模型,应激结束后进行Morris水迷宫实验检测小鼠应激状态下的学习记忆能力,并采用放射免疫方法检测小鼠脑组织中NPY的含量。结果应激组小鼠在Morris水迷宫中寻找平台的潜伏期较对照组显著延长(P<0.01),在平台所在象限的游泳时间比例显著小于对照组(P<0.01)。与对照组相比,应激组小鼠脑组织NPY的表达显著增加(P<0.01)。结论慢性应激可引起机体的学习记忆能力的改变,主要表现为注意力不集中、记忆力减退;慢性应激可导致小鼠脑组织NPY的高表达,且NPY与心理应激引起小鼠学习记忆能力的改变相关,提示NPY可能作为应激分子参与机体的心理应激过程。Objective This study adopted immobilization stress model in order to observe the effect of chronic immobilization stress on change of learning and memory and neuropeptide Y in mice. Methods The mice mode of stress was made by restraint for 6 hours per day. Morris water maze test was performed on change of function of learning and memory in mice. RIA was employed to measure levels of neuropeptide Y in brain. Results In Morris water maze,the mean escape latency of the mice in the chronic re- straint groups were longer than that of the control mice（P〈0.01）. The average percentages of the swimming time in the platform quadrant in the restraint group mice were less than those in the control group （P〈0. 01）. Neuropeptide Y expressions in brain markedly increased in chronic immobilization stress（P〈0. 01）. Conclusions Chronic psychological stress can lead to learning and memory decline. Chronic immobilization stress could induce neuropeptide Y increases and NPY correlate with the changes of learn- ing and memory in mice. It suggests that NPY is viewed as stress molecular and participate the process of psychological stress.

关 键 词：慢性应激 学习记忆 神经肽Y 

分 类 号：R285.5[医药卫生—中药学] R963[医药卫生—中医学]