平阳霉素(Pingyangmycin)对小麦诱变效应的研究  

STUDIES ON THE MUTAGENIC EFFECTS OF PINGYANGMYCIN ON WHEAT

作　　者：朴铁夫[1] 原亚萍[1] 李国全[1] 于少华[1] 李梦[1] 孙忠农 许耀奎[1] 包和平[1] 

机构地区：[1]吉林农业大学

出　　处：《核农学报》1998年第3期139-145,共7页Journal of Nuclear Agricultural Sciences

基　　金：吉林省科委应用基础科研课题

摘　　要：本试验研究了平阳霉素（Ｐｉｎｙａｎｇｍｙｃｉｎ）对小麦的诱变效应。用平阳毒素１０μｇ／ｍｌ、３０μｇ／ｍｌ和５０μｇ／ｍｌ浓度处理小麦种子，Ｍ１根长平均数分别为２００６ｃｍ、１７８４ｃｍ和１７０７ｃｍ，３种浓度平阳霉素处理均比０３％ＥＭＳ处理抑制根长的效应显著；平阳霉素１０μｇ／ｍｌ、３０μｇ／ｍｌ和５０μｇ／ｍｌ３种浓度处理的根数平均数分别为４５９、３９７和３５３。平阳霉素处理的根数平均数明显少于对照和０３％ＥＭＳ处理；平阳霉素１０μｇ／ｍｌ、３０μｇ／ｍｌ和５０μｇ／ｍｌ３种浓度处理的Ｍ１苗高平均数分别为７３９ｃｍ、６９３ｃｍ和６６９ｃｍ，比对照苗高有明显降低。平阳霉素１０μｇ／ｍｌ、３０μｇ／ｍｌ和５０μｇ／ｍｌ浓度处理，出苗率分别为７３％、６９％和６６％，Ｍ１根尖细胞染色体畸变率分别为６１５％、７３１％和５１７％，Ｍ２突变率分别为２２５％、２５５％和３８３％；平阳霉素处理比对照和甲基磺酸乙酯（ＥＭＳ）处理，对Ｍ１出苗率及根尖细胞染色体畸变率都有明显的影响，试验结果表明平阳霉素是一种具有较强诱变效应的新诱变剂。The mutagenic effect of Pingyangmycin on wheat was studied in this experiment and average root lengths of wheat plant treated by Pingyangmycin were 20 06cm,17 84cm and 17 07cm,respectively.lnhibiting effects on root length were stronger when treated by three concentration of Pingyangmycin than that with EMS treatment.Average root number of wheat treated by Pingyangmicin of 10μg/ml,30μg/ml and 50μg/ml were 4 59,3 97 and 3 53 respectively,obviously fewer than CK and 0 3% EMS treatment.M 1 seedling heights,were 7 39cm,6 93cm and 6 69cm when treated by Pingyangmycin of 10μg/ml,30μg/ml,50μg/ml,respctively,obviously lower than CK.Seedling emerging rates were 70%,69%,66%, when treated by Pingyangmycin of 10μg/ml, 30μg/ml,50μg/ml,respectively.Chromosomal aberration rates were 6 15%,7 36% and 5 18%, respectively.Mutation frequencies of M 2 were 2 25%,2 55% and 3 83%, respectively.The results indicate that Pingyangmycin has strong mutagenic effect and can be used as a new mutagenic agent.

关 键 词：平阳霉素 小麦 诱变效应 

分 类 号：S512.1[农业科学—作物学]