高效液相色谱法测定醋酸曲安奈德尿素乳膏中醋酸曲安奈德的含量  

Content determination of Triamcinolone in Triamcinolone Urea Cream by HPLC

作　　者：胡守莲 范玉峰[2] 李道明 

机构地区：[1]河南省信阳市食品药品检验所,河南信阳464000 [2]信阳职业技术学院附属医院,河南信阳464000

出　　处：《中国医药导报》2009年第27期53-54,64,共3页China Medical Herald

摘　　要：目的:建立醋酸曲安奈德尿素乳膏中醋酸曲安奈德含量的HPLC测定方法。方法:用Suntek Kromasil-C18(4.6mm×200mm,5μm)色谱柱;流动相:甲醇-水(75∶25);流速:1.0ml/min;检测波长:240nm;柱温:30℃。结果:醋酸曲安奈德在6.25～50.00μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.60%,RSD=0.40%(n=9)。结论:该法简便、准确、重现性好,可用于醋酸曲安奈德尿素乳膏中醋酸曲安奈德的质量控制。Objective：To establish a method for the content determination of Triamcinolone in Triamcinolone Urea Cream by HPLC.Methods：The Suntek Kromasil-C18（4.6 mm×200 mm,5 μm）column was used.The mobile phases consisted of methanol and water（75：25）,the flow rate was 1.0 ml/min and the detection wavelength was at 240 nm.The column temper-ature was 30℃.Results：The linear ranges of Triamcinolone was at 6.25-50.00 μg（r=0.999 9）,the average recovery was 99.60% with a RSD of 0.40%（n=9）.Conclusion：The method is fast,reliable,accurate and can be used in the quality con-trol of Triamcinolone in Triamcinolone Urea Cream.

关 键 词：醋酸曲安奈德尿素乳膏 醋酸曲安奈德 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]