利多卡因对脂多糖诱导的巨噬细胞高迁移率族蛋白B1 mRNA表达的影响  

Effects of lidocaine on expression of HMGB1 mRNA induced by lipopolysaccharide in rat peritoneal macrophages

作　　者：周长青[1] 王焕亮[1] 张丽[1] 李建军[1] 王雪芹[1] 

机构地区：[1]山东大学齐鲁医院第二麻醉科,济南市250012

出　　处：《临床麻醉学杂志》2009年第9期792-794,共3页Journal of Clinical Anesthesiology

基　　金：山东省自然科学基金(项目编号:Y2007C115)

摘　　要：目的观察利多卡因对脂多糖(LPS)诱导的大鼠腹腔巨噬细胞高迁移率族蛋白B1(HMGB1)mRNA表达的影响。方法取雄性Wistar大鼠腹腔巨噬细胞,置6孔细胞培养板中培养3～5 d后,分为四组:脂多糖组(LPS组),LPS刺激浓度为100 ng/ml;L1组,于LPS刺激前30 min给予利多卡因(终浓度为20μg/ml)预处理;L2组,于LPS刺激后30 min给予同等剂量的利多卡因处理;L3组分别于LPS刺激后30 min、6、12、18 h给予同等剂量的利多卡因处理。LPS刺激24 h后收取细胞,采用RT-PCR检测细胞HMGB1 mRNA的表达。结果与LPS组比较,L1、L2、L3组HMGB1 mRNA表达均有不同程度下降(P<0.01)。L3组HMGB1 mRNA表达下降程度最大,明显低于L1、L2组(P<0.01)。结论利多卡因能够抑制LPS诱导的体外培养大鼠腹腔巨噬细胞HMGB1 mRNA的表达。Objective To investigate the effects of lidocaine on expression of high mobility group box-1 （HMGB1） protein mRNA induced by lipopolysaccharide （LPS） in rat peritoneal macrophages. Methods Peritoneal maerophages obtained from male Wistar rats were incubated in sixwell tissue culture plates for 3-5 days before stimulated with LPS and treated with lidocaine. The rats were divided into 4 groups of LPS, L1 ,L2 and L3. After washing with serum-free RPML1640 culture medium,all macrophages were incubated with 2.0 ml serum free RPMI-1640 culture medium. The macrophages in group LPS were stimulated with LPS in a concentration of 100 ng/ml, those in group L1 with lidocaine in a concentration of 20 μg/ml 30 minutes before LPS stimulation, those in group L2 with lidocaine in the same doses 30 minutes after LPS stimulation, and those in group L3 with lidocaine in the same doses at 30 min, 6 h, 12 h, 18 h after LPS stimulation. Then all macrophages were incubated for 24 hours after LPS stimulation. The expression of HMGB1 mRNA in the macrophages were detectned by RT-PCR. Results Compared to group LPS, the expression of LPS-induced HMGB1 mRNA was significantly inhibited in a certain degree by lidocaine in groups of L1, L2 and L3, which was more in group L3 than that in group L2 or L1 （P〈0.01）. Conclusion Lidocaine can significantly inhibit the expression of HMGB1 mRNA induced by LPS in rat peritoneal macrophages.

关 键 词：利多卡因 巨噬细胞 高迁移率族蛋白B1 

分 类 号：R96[医药卫生—药理学]