海南橡胶藻18S rDNA与16S rDNA二级结构构建及分析  

The Secondary Structure Model Building and Analysing of Heveochlorella hainangensis sp. nov.

作　　者：常凯军[1] 谭德冠[1] 李定琴[1] 孙雪飘[1] 张家明[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室,海南海口571101

出　　处：《安徽农业科学》2009年第27期12965-12967,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]对海南橡胶藻的新属、新种地位进行确认。[方法]对海南橡胶藻这一重要生物资源的18S核编码核糖体RNA序列和16S叶绿体编码核糖体RNA序列进行分析,对18S核编码核糖体RNA进行近缘物种间的序列比对及2个I类内含子二级结构模型的构建。[结果]橡胶藻16S rRNA在639位点(E.coli序列编号)有一个46 bp的插入片段,该片段能形成一个完美的双发夹结构,对16SrRNA的正常折叠不会产生影响。[结论]这一双发夹结构在其他物种的叶绿体rDNA中还未见报道过。[ Objective ] The research aimed to affirm the new category and new species status of Heveochlorella hainangensis sp. nov.. [ Method ] The nuclear encoded 18S rDNA and the nuclear encoded 16S rDNA were analyzed. The nuclear sequence between H. hainangensis and the nearly relationship varieties were contrasted. And the secondary structure model of two group I intron was built. [ Result] Alignment analysis revealed a unique feature of 16S rDNA with an insertion of 46 bp at position 639 （E. coli numbering） in helix P21-1. This fragment formed a perfeet double hairpin structure. [ Conclusion] This structure had not been found in the plastid of any other species reported, including higher plants.

关 键 词：18S核编码核糖体RNA 16S叶绿体编码核糖体RNA I类内含子 双发夹结构 

分 类 号：S335.4[农业科学—作物遗传育种]