中国荷斯坦牛AGPAT6基因第2内含子PCR-SSCP分析  

PCR-SSCP Analysis on Intron 2 of AGPAT6 Gene in Chinese Holstein

作　　者：张佳兰[1] 昝林森[2] 

机构地区：[1]长江大学动物科学学院,湖北荆州434025 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《安徽农业科学》2009年第27期12970-12971,13075,共3页Journal of Anhui Agricultural Sciences

基　　金：长江大学博士启动基金项目

摘　　要：[目的]寻找影响奶牛产奶性能的候选基因。[方法]以中国荷斯坦牛为研究对象,采用PCR-SSCP方法对AGPAT6基因第2内含子进行单核苷酸多态性检测并分析多态位点对奶牛产奶量、乳脂率、乳蛋白率和乳中体细胞评分的影响。[结果]扩增出462 bp的中国荷斯坦牛AGPAT6基因第2内含子;PCR-SSCP分析该基因第2内含子有AA、AB和BB 3种基因型,其频率分别为0.151 1、0.657 8和0.191 1;不同基因型与中国荷斯坦牛产奶量、乳脂率和乳蛋白率之间显著相关(P<0.05),与体细胞评分相关不显著(P>0.05)。[结论]初步认为AGPAT6是影响奶牛产奶性能的一个候选基因或分子标记,该研究为奶牛选育提供了理论依据。[ Objective ] The aim was to search for the candidate gene for influencing milk performance of dairy cow. [ Method ] With Chinese Holstein as research object, the single nucleotide polymorphism of intron 2 of AGPAT6 gene was detected by PCR-SSCP and the effect of polymorphic loci on milk yield, milk fat percentage, milk protein percent and somatic cell score of dairy cow were analyzed. [ Result] The 462 bp intron 2 of AGPAT6 gene of Chinese Holstein was amplified, which could be used for PCR-SSCP analysis. There were 3 genotypes AA, AB and BB in intron 2 ofAGPAT6 gene and their genotype frequency were 0. 151 1,0. 657 8 and 0. 191 1, resp. There was significant relation between different genotypes in milk yield, milk fat percentage, milk protein percentage （ P 〈 0.05 ） and no significant relation in somatic cell score （ P 〈 0.05 ）. [ Conclusion ] The results preliminary showed that AGPAI^5 gene was either a candidate gene influencing milk performance of dairy cow or a molecular marker. The research provided the theoretical basis for dairy cow breeding.

关 键 词：AGPAT6基因 中国荷斯坦牛 PCR-SSCP分析 

分 类 号：S823[农业科学—畜牧学]