新三黄合剂的质量标准研究  

Study on the Quality Standard of New Sanhuang Mixture

作　　者：尹健平 

机构地区：[1]邵东县人民医院,湖南邵东422800

出　　处：《中医药导报》2009年第9期55-58,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立新三黄合剂的质量标准。方法:用TLC法对大黄、丹参、姜黄、水蛭进行定性鉴别;用HPLC法测定大黄中大黄酸、大黄素和大黄酚的含量。结果:薄层色谱图斑点清晰,阴性对照无干扰;大黄酸在0.0382-0.4202μg范围内线性关系良好,平均回收率98.53%,RSD%为0.75%(n=6),大黄素在0.0164-0.1804μg范围内线性关系良好,平均回收率99.41%,RSD%为1.31%(n=6),大黄素在0.0204-0.2244μg范围内线性关系良好,平均回收率97.96%,RSD%为1.65%(n=6)。结论:该方法专属性强,灵敏度高,重现性好,可用于新三黄合剂的质量控制。Objective： To establish the quality standard study for New Sanhuang Mixture. Methods： Radix et Rhizoma Rhei, Radix et Rhizoma Salviae mihiorrhizae, Rhizoma Curcumae longae,Hirudo in New Sanhuang Mixture were identified by TLC; The content of Rhein,emodin and Chrysophanol was determined by HPLC. Results： The TLC sports developed was fairly clear, and the blank test showed no interference. The calibration curve of Rhein was linear in the range of 0.0382-0.4202 μg, its average recovery was 98.53% and RSD% was 0.75%（n=6）.The calibration curve of emodin was linear in the range of 0.0164-0.1804μg, its average recovery was 99.41% and RSD% was 1.31%（n=6）. The calibration curve of Chrysophanol was linear in the range of 0.0204-0.2244 μg, its average recovery was 97.96% and RSD% was 1.65% （n =6）. Conclusion： The selective,sensitive and reproducibility of the method are good, the method can be used for quality of control of New Sanhuang Mixture.

关 键 词：新三黄合剂 大黄酸 大黄素 大黄酚 TLC HPLC 

分 类 号：R282.5[医药卫生—中药学] R927.4[医药卫生—中医学]