成都地区肥胖人群UCP2基因多态性研究及其与肠道菌群关系的初探  

Study on Polymorphism of UCP2 Gene in Chengdu Simple Obesity and Normal-weight People and a Preliminary Investigation of Its Relationship with Gut Bacteria

作　　者：左浩江[1] 张薇薇[1] 谢志梅[1] 李永儒[1] 王嵬[1] 丁晓贝[1] 裴晓方[1] 

机构地区：[1]四川大学华西公共卫生学院医学检验学教研室,成都610041

出　　处：《四川大学学报（医学版）》2009年第5期865-868,876,共5页Journal of Sichuan University(Medical Sciences)

基　　金：达能膳食营养研究与宣教基金(06HT25)资助

摘　　要：目的研究成都地区单纯性肥胖和正常体质量成人解偶联蛋白2基因(UCP2)Ala55Val多态性,初步探讨UCP2基因Ala55Val不同基因型与肠道菌群关系。方法采用PCR-RFLP检测86例成都地区成人(单纯性肥胖组43例,正常体质量组43例)的UCP2基因ALa55Val多态性,并分别比较不同基因型间6种肠道菌群数量的差异。结果单纯性肥胖组和正常体质量组均存在UCP2基因Ala/Ala、Ala/Val、Val/Val多态,两者表型分布差异有统计学意义(χ2=11.97,P<0.05);单纯性肥胖组UCP2等位基因突变频率高于正常体质量组,差异有统计学意义(χ2=10.06,P<0.05);单纯性肥胖组、正常体质量组和研究总体中不同基因型人群的6种肠道菌群数量差异无统计学意义(P>0.05)。结论UCP2基因ALa55Val变异可能是成都地区人群单纯性肥胖的危险因素,但该基因ALa55Val变异与肥胖的关联可能与肠道菌群数量改变无关。Objective To study on the polymorphism of UCP2 gene in Chengdu simple obesity and normalweight people and to initially investigate the relationship between UCP2 Ala55Val variation and gut bacteria. Methods PCR-PFLP was applied to determine the genotypes of Ala55Val variant in the UCP2 gene of 86 Chengdu people （the simple obesity group, 43 subjects; the normal-weight group, 43 subjects）. And six kinds of gut bacteria among different genotypes in different groups were analyzed. Results Both the simple obesity and the normalweight group had the Ala55Val variants of Ala/Ala, Val/Val and Ala/Val in the UCP2 gene, and the Ala55Val genotype distributions between the two groups was significantly different （χ^2 = 11. 97, P〈 0. 05）. The allelic mutation frequency in the simple obesity group was higher than that of the normal-weight group （χ^2 = 10.06,P〈 0. 05）. However, no significant difference was observed in the population of six gut bacteria among different genotypes in different groups （P〉0.05）. Conclusion The UCP2 gene mutation might be a risk factor of obesity in Chengdu area. However, this gene mutation may not be an impact factor on the alternation of gut bacteria.

关 键 词：单纯性肥胖 解偶联蛋白2基因 基因多态性 肠道菌群 

分 类 号：R589.2[医药卫生—内分泌]