LA0202为钩端螺旋体中一孔形成溶血素蛋白(英文)  

A novel pore-forming hemolysin protein in Leptospira species

作　　者：杨杨[1] 秦金红[1] 钟怡[1] 何平[1] 胡宝瑜[1] 郭晓奎[1] 

机构地区：[1]上海交通大学医学院病原生物学教研室,上海200025

出　　处：《中国人兽共患病学报》2009年第9期831-836,共6页Chinese Journal of Zoonoses

基　　金：supported in part by the grants from the National Natural Science Foundation of China (No.30670102 & 30770111 & 30770820);the National High Technology Research and Development Program of China (2006AA02Z176);Shanghai Natural Science Foundation (06ZR14056)

摘　　要：目的研究LA0202为孔形成溶血素。方法体外克隆表达LA0202蛋白。纯化的LA0202蛋白作用绵羊血细胞观察LA0202的溶血活性并用渗透压保护剂PEG6000添加到作用体系研究渗透压保护剂对LA0202溶血活性的影响。透射电镜及扫描电镜观察LA0202作用于体外培养的肝细胞后对细胞的毒性。结果渗透压保护剂PEG6000能够抑制LA0202的溶血活性。LA0202作用于肝细胞后引起肝细胞的毒性损伤。结论LA0202为一孔形成溶血素。Hemolysins of Leptospira interragans have been shown to be the virulence factor in the pathogenesis of leptospirosis and 10 potential hemolysin genes were charecterized by genomic annotation of L. interrogans serovar. Lai strain 56601. In the present study, the LA0202 gene supposed to encode one of the new potential hemolysin was cloned and the protein encoded was purified. The purified protein was shown to have highly hemolytic activity as demonstrated on the sheep blood agar plate. It was also confirmed that the LA0202 protein mediated hemolysis on sheep erythrocytes was osmotically protected by PEG6000. Meanwhile, this protein could induce pore formation on sheep erythrocytes and cause damages on the membrane of human L-02 liver cells. In addition, it could induce apoptosis of human L-02 liver cells after treatment of cells with this protein for 24 hours. It is evident that LA0202 protein acting as a pore-formong hemolysin can induce eytotoxic damage on mammalian cells.

关 键 词：溶血素 钩端螺旋体 蛋白 PEG6000 溶血活性 扫描电镜观察 细胞观察 体外培养 

分 类 号：R285.5[医药卫生—中药学] R377.5[医药卫生—中医学]