高胆固醇摄入对新西兰兔胆汁酸代谢的影响  

Effects of dietary cholesterol intake on bile acids metabolism in New Zealand white rabbits

作　　者：邱冬妮[1] 孙旭[1] 钟良[1] 毛奇琦[1] 陈坚[1] 蒋蔚茹[1] 黄剑平[1] 孙大裕[1] 

机构地区：[1]复旦大学附属华山医院消化科,上海200040

出　　处：《复旦学报（医学版）》2009年第5期601-604,共4页Fudan University Journal of Medical Sciences

摘　　要：目的观察2周高胆固醇食物摄入对新西兰兔胆汁酸经典合成途径基因调节的影响。方法20只新西兰兔随机分为对照组和高胆固醇组,分别喂饲普通兔粮及高胆固醇兔粮2周,测定实验前后血清总胆固醇(TC)、低密度脂蛋白(LDL)、胆汁酸(bile acid,BA)及肝脏胆固醇7α羟化酶(cholesteral 7-αhydroxylase,CYP7A1)活性及mRNA、小分子异源二聚体伴侣(short heterodimer partner,SHP)mRNA、胆盐输出泵(bile saltexport pump,BSEP)mRNA和三磷酸腺苷结合盒转运蛋白A1(ATP-binding cassette transporter A1,ABCA1)mRNA、胆固醇酯转移蛋白(cholesteryl-ester transfer protein,CETP)mRNA。结果高胆固醇组TC、LDL、BA均较对照组升高(P<0.05);高胆固醇组CYP7A1活性及mRNA均较对照组降低(P<0.05);高胆固醇组SHPmRNA、BSEP mRNA均较对照组升高(P<0.05);胆固醇组ABCA1 mRNA、CETP mRNA均较对照组升高。结论高胆固醇摄入后肝脏FXR、LXR受体均被激活,CYP7A1活性下降,胆汁酸经典合成减少。Objective To detect the effect of 2 synthesis pathway in New Zealand white rabbits. week dietary cholesterol intake on bile acids classic divided into two groups randomly： control group Methods Twenty New Zealand white rabbits were and cholesterol group. Each group contained 10 rabbits. The control group was fed regular forage, and the cholesterol group was fed 2% cholesterol forage. Serum TC, LDL, bile acid （BA）, hepatic cholesteral 7α-hydroxylase （CYP7A1） activity and mRNA, short heterodimer partner （SHP） mRNA and bile salt export pump （BSEP） mRNA, ATP- binding cassette transporter A1 （ABCA1） mRNA and cholesteryl-ester transfer protein （CETP） mRNA were measured. Results Serum TC, LDL and BA were higher in cholesterol group compared with those in control group （P〈0.05）. Hepatic CYP7A1 activity and mRNA in cholesterol group were higher than those in the control group （P〈0. 05）. Hepatic SHP mRNA, BSEP mRNA were elevated in cholesterol group compared with those in the control group （P〈0.05）. Hepatic ABCA1 mRNA and CETP mRNA were elevated in cholesterol group compared with those in the control group （P〈0. 05）. Conclusions Dietary cholesterol intake activates hepatic nuclear receptor FXR and LXR, decreases CYP7A1 activity and bile acid classic systhesis.

关 键 词：胆汁酸 胆固醇 法尼酯衍生物X受体 肝脏X受体 胆固醇7α羟化酶 兔 

分 类 号：Q548.5[生物学—生物化学]