醋酸曲普瑞林对子宫腺肌病在位内膜细胞VEGF、TNF-α分泌的影响  

Effect of Triptorelin on release of VEGF and TNF-α in endometrial cells from patients with adenomyosis

作　　者：禤坚艳[1,2] 康佳丽[1] 王小霞[1] 余佳[1] 聂妙玲[1] 邓玲红[1] 王冬昱[1] 

机构地区：[1]广州医学院附属广州市第一人民医院妇产科,510180 [2]广东省佛山市妇幼保健院

出　　处：《中国妇产科临床杂志》2009年第5期356-359,共4页Chinese Journal of Clinical Obstetrics and Gynecology

基　　金：广东省科技计划项目(2006B35930003)资助

摘　　要：目的探讨醋酸曲普瑞林对子宫腺肌病在位内膜细胞血管内皮生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)分泌的影响。方法取子宫腺肌病患者在位内膜细胞及对照组正常子宫内膜细胞进行体外培养,并给予不同浓度醋酸曲普瑞林处理后采用ELISA法检测细胞培养上清液中VEGF、TNF-α的含量。结果VEGF、TNF-α在子宫腺肌病在位内膜细胞[(213.97±32.51)pg/ml]中的分泌均高于正常子宫内膜细胞[(119.54±17.32)pg/ml](P<0.05);醋酸曲普瑞林呈剂量依赖方式抑制子宫腺肌病在位子宫内膜细胞及正常子宫内膜细胞VEGF、TNF-α的分泌(P<0.05)。结论VEGF、TNF-α在子宫内膜中的异常分泌可能与子宫腺肌病的发生发展有关;醋酸曲普瑞林可直接作用于在位子宫内膜细胞,阻碍血管生成,从而抑制子宫内膜的异位种植和生长。Objective To investigate the effect of Triptorelin on the release of tumor necrosis factor-α （TNF -α） and vascular endothelial growth factor （VEGF） in the in - vitro eutopic endometrial cells of adenomyosis. Methods Biopsy specimens of eutopic endometrium obtained from 20 women with adenomyosis and 16 normal women were studied. Cells were cultured with Triptorelin in the concentration of 10-7 mol/L and 10-5 mol/L TNF-α and VEGF concentrations in culture supernatant were detected by commercial enzyme- linked immunosorbent assay （ELISA）. Results The concentrations of VEGF and TNF-α in the eutopic endometrial cells of adenomyosis were significantly higher than those in the normal endometrium. The concentrations of VEGF, TNF -α in the both groups were downregulated by Triptorelin in a dose - dependent manner. Conclusion VEGF and TNF-α may play an impotant role in the pathogenesis of adenomyosis. Triptorelin can directly suppress the survival and growth of ee- topic endometrial by decreasing the release of VEGF and TNF-α which are related to the adenomyosis angiogenesis.

关 键 词：子宫腺肌病 子宫内膜细胞 促性腺激素释放激素激动剂 血管内皮生长因子 肿瘤坏死因子-α 

分 类 号：R711.71[医药卫生—妇产科学] R711.74[医药卫生—临床医学]