N-乙酰氨基葡萄糖激活Ca^(2+)信号通路诱导小鼠T淋巴细胞增殖  

N-acetyl-D-glucosamine induces proliferation of the mouse T lymphocytes through Ca^(2+) signal pathway

作　　者：曹秀明[1] 高越[1] 马娇[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物所抗肿瘤天然药物教育部工程研究中心,哈尔滨150076

出　　处：《现代免疫学》2009年第5期376-380,共5页Current Immunology

基　　金：黑龙江省科技厅自然科学基金资助项目(D2007-45)

摘　　要：研究N-乙酰氨基葡萄糖体外作用于小鼠T淋巴细胞诱导淋巴细胞增殖的机制。应用Ca2+探针(Fura-3/AM)、免疫荧光法分析Ca2+信号通路。结果:N-乙酰氨基葡萄糖能够使T淋巴细胞内的Ca2+浓度增加,钙调蛋白和钙调磷酸酶的表达增加。N-乙酰氨基葡萄糖是一种新的通过激活淋巴细胞Ca2+信号通路诱导T淋巴细胞增殖的活化剂。To investigate the mechanism of T lymphocyte proliferation induced by N-acetyl-D-glucosamine in vitro, the Ca^2＋ signal pathway was analyzed by using Ca^2＋ fluorescent probe （Fura-3/AM） and immunofluorescence method . It was dem- onstrated that N-aeethyl-D-glucosamine induced the increase of Ca^2＋ concentration in T lymphocytes and the expressions of CaM and CaN. Indicating it is a new kind of activator, which can induce proliferation of T lymphocytes through Ca^2＋ signal pathway.

关 键 词：N-乙酰氨基葡萄糖 淋巴细胞增殖 CA^2+ 钙调蛋白 钙调磷酸酶 

分 类 号：R392.12[医药卫生—免疫学]