银屑病患者骨髓基质细胞IL-1α、IL-1β和IL-11分泌水平的实验研究  

Experimental study on the levels of IL-1α、IL-1β and IL-11 secreted by bone marrow stromal cells of patients with psoriasis

作　　者：尹国华[1] 刘瑞风[1] 张开明[1] 

机构地区：[1]太原市中心医院皮肤科,太原030009

出　　处：《现代免疫学》2009年第5期405-408,共4页Current Immunology

基　　金：国家自然科学基金资助项目(30771940)

摘　　要：通过比较银屑病患者与对照组骨髓基质细胞分泌IL-1α、IL-1β和IL-11水平的差异,揭示银屑病患者骨髓造血微环境的异常。采用密度梯度离心法分离患者与对照组骨髓单个核细胞,通过贴壁法培养骨髓基质细胞,收集传3代后又培养72 h的骨髓基质细胞及培养上清,用流式细胞术鉴定细胞表面标志并用ELISA法检测上清液中IL-1α、IL-1β和IL-11的分泌水平。结果显示:90%以上细胞表面抗原高表达CD29,而CD34、CD45及HLA-DR表达阴性,即骨髓基质细胞纯度在90%以上;患者组骨髓基质细胞分泌IL-1α、IL-1β显著低于对照组(P<0.05),而IL-11的分泌水平两组间无统计学差异(P>0.05)。结果表明,银屑病患者骨髓基质细胞分泌的IL-1α和IL-1β存在异常,提示患者骨髓造血微环境可能存在异常。To reveal the abnormity of bone marrow microenvironment of patients with psoriasis by comparing the level of IL- 1α, IL-1β and IL-11 secreted by bone marrow stromal cells of patients and control individuals, separate bone marrow mononu- clear cells of patients and controls were isolated by density gradient centrifugation, and the bone marrow stromal cells were cultivated by the adherence method. When the cells were cultured for 3 passages and another 72 hours, bone marrow stromal cells and the supernatant liquid were collected, the phenotypes of cells were identified by flow cytometry and the level of IL-1α, IL- 1β and IL-11 were assayed by ELISA kits. The results showed that the purity of bone marrow stromal cells was over 90%, and the level of IL-1α and IL-1β secreted by bone marrow stromal cells of patients was lower than that of control（P〈0. 05）. However, the level of IL-11 showed no significant difference between these two groups（P〉0.05）. It is concluded that abnormality exists in the secretion of IL-α and IL-β by bone marrow stromal cells in patients with psorriasis, indicating an abnormal bone marrow microenviorment in this kind of patients.

关 键 词：银屑病 骨髓基质细胞 IL-1Α IL-1Β IL-11 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]