参附注射液对创伤失血性休克大鼠的肾保护作用研究  

A study on expressions of tissue factor mRNA and thrombomodulin mRNA in kidney of rats with hemorrhagic shock and interference effects of Shenfu injection （参附注射液） for resuscitation

作　　者：马继红[1] 刘婷[2] 潘景业[1] 陈洁[1] 张近波[1] 

机构地区：[1]温州医学院附属第一医院ICU,浙江温州325000 [2]中国医科大学北京顺义医院ICU,北京101300

出　　处：《中国中西医结合急救杂志》2009年第5期293-295,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：基金项目：浙江省温州市科技计划项且（Y20060062）

摘　　要：目的探讨失血性休克大鼠肾组织内皮细胞组织因子（TF）、血栓调节蛋白（TM）的mRNA表达及参附注射液的干预作用。方法将40只SD大鼠随机分成对照组、休克组、林格液组、参附液组，每组10只。采用放血法制备失血性休克大鼠模型。林格液组和参附液组于制模后分别用林格液或参附注射液（10ml／kg）加林格液复苏。制模各组于复苏后2h、对照组于置管后3h取肾组织，用逆转录-聚合酶链反应（RT—PCR）检测TF、TM的mRNA表达。结果休克组TF、TM的mRNA表达较其他组均显著升高（P均〈0．01）；而对照组则较其他组均显著降低（P均〈0．01）。参附液组TFmRNA表达较休克组和林格液组均显著下降（P〈0．01和P〈0．05），而TMmRNA表达则显著高于林格液组（P〈0．05）。结论失血性休克后存在肾组织及内皮细胞损伤；参附注射液从促凝与抗凝两个方面保护肾组织，从而对机体凝血功能产生影响。Objective To investigate the changes of the expressions of tissue factor （TF） mRNA and thrombomodulin （TM） mRNA in endothelial cells of kidney tissues in rats with hemorrhagic shock （HS） and the interference effects of Shenfu injection （SFI, 参附注射液）. Methods Forty adult healthy Sprague-Dawley （SD） rats were randomly assigned to the following four groups （each n= 10） ： wound control （WC group）, HS group, resuscitation with Ringerts solution （RS group） and resuscitation with SFI group （SFI group）. The rat model of HS was established by blood letting. In the WC group, the rat was subjected to insertion of catheter respectively into the right carotid artery and left femoral vein only without any other management; after the model establishment, Ringer＇s solution was used in RS group and RS plus SFI （10 ml/kg） mixture was applied in the SFI group for resuscitation. At 2 hours after resuscitation and 3 hours after catheterization in WC group, the kidney tissue was collected. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expressions of TF mRNA and TM mRNA. Results The expressions of TF mRNA and TM mRNA in HS group were significantly higher than those in other groups （all P〈0.01） ; while the expressions in WC group were obviously lower than those in other groups （all P〈0.01）. The expression of TF mRNA in SFI group was markedly lower than that in HS group and RS group （P〈0.01 and P〈0. 05）, while the expression of TM mRNA in SFI group was significantly higher than that in RS group （P〈0.05）. Conclusion After the occurrence of HS in rats, there is kidney tissue and endothelial cell injury. SFI can protect renal tissues from both pro- and anti-coagulant aspects; its affect on the production and release of TF and TM in kidney may have an impact on the coagulation of an organism.

关 键 词：失血性休克 组织因子 血栓调节蛋白 参附注射液 

分 类 号：R285.5[医药卫生—中药学] R605.971[医药卫生—中医学]