酶法拆分N-乙酰-D,L-蛋氨酸转化条件优化  

Optimization of Operation Conditions of Split N-Acetyl-D,L-Methionine by Enzymatic Method

作　　者：李环[1] 金圣芳[1] 高浩峰[1] 韦萍[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009

出　　处：《化学反应工程与工艺》2009年第4期329-333,共5页Chemical Reaction Engineering and Technology

基　　金：国家973项目基金资助(2009CB724702)

摘　　要：重组大肠杆菌BL21/pET22b-argE表达的N-乙酰鸟氨酸脱酰基酶(NAOase)可用于脂肪族氨基酸的手性拆分,水解N-乙酰-D,L-氨基酸中的L-型底物,N-乙酰-D,L-蛋氨酸为其最合适的底物。为了确定NAOase拆分N-乙酰-D,L-蛋氨酸合适的转化条件,考察了反应温度、pH值、Co^(2+)浓度、转化时间、底物浓度和加酶量对产物的影响。结果表明,合适的反应条件为37℃,pH值7.0,Co^(2+1) mmol/L,转化时间20 min,底物浓度150 mmol/L,菌泥5 g/L。在上述反应条件下,N-乙酰-D,L-蛋氨酸的转化率可达81.4%。N-acety-L-ornithine deacetylase （NAOase） expressed by recombinant engineering strain BL21/pET22b-argE can be used to separate chiral aliphatic amino acids, and its optimal substrate is N-acetyl-D,L-methionine. In order to obtain the appropriate conversion conditions of N-acetyl-D,L -methionine by NAOase, the effects of temperature, pH, concentration of Co^2＋, concentration of substrate and enzyme dosage on conversion of N-acetyl-D,L-methionine were investigated. The results showed that the conversion of N-acetyl-D,L conditions of 37 ℃, pH7.0, concentration of Co^2＋ 1 of substrate 150 mmol/L and enzyme 5 g/L.

关 键 词：N-乙酰鸟氨酸脱酰基酶 N-乙酰-D L-蛋氨酸 酶法拆分 转化条件 

分 类 号：Q814.9[生物学—生物工程]