HPLC法研究当归-红花单煎及共煎液中有效成分含量的变化  

Study on Content Variation of Active Ingredient in Single and Mixed Decoction of Radix Angelica Sinensis and Flos Carthami by HPLC

作　　者：张荣[1] 刘睿[1] 黄天来[1] 宓穗卿[1] 王宁生[1] 

机构地区：[1]广州中医药大学临床药理研究所,广州510405

出　　处：《中药新药与临床药理》2009年第5期448-452,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省高等学校中药有效性与安全性重点实验室开放基金(kf07002)

摘　　要：目的研究和探讨当归-红花单煎及共煎液中有效成分阿魏酸和羟基红花黄色素A含量的变化,初步探索两味中药配伍应用的变化规律。方法采用Phenomenex C18柱(4.6mm×150mm,5μm),以甲醇-0.05%磷酸水溶液p(H=2.45,25∶75)为流动相;流速:1.0mL/min;柱温:30℃;进样温度:5℃;检测波长分别为320nm、403nm,测定追踪当归-红花共煎液与单煎液中主要有效成分阿魏酸和羟基红花黄色素A含量的变化。结果阿魏酸回归方程为Y=3.8372X+0.0219(r=0.9998),在0.5～10.0μg mL/范围内线性关系良好,平均加样回收率为92.68～101.13%,RSD小于3.2%;羟基红花黄色素A回归方程为Y=1.3647X-0.3714(r=0.9999),在5.0～100.0μg mL/范围内线性关系良好,平均加样回收率为100.17～102.97%,RSD小于2.8%;当归红花共煎液中阿魏酸含量比当归单煎液中平均升高142.64%,羟基红花黄色素A含量比红花单煎液中平均升高145.37%。结论当归-红花共煎液中阿魏酸和羟基红花黄色素A的含量高于各单煎液中含量,两者配伍具有一定的合理性。该法快速、简便、准确,可为评价提取物中阿魏酸和羟基红花黄色素A的质量提供依据。Objective To study the changes of the contents of ferulic acid and hydroxysafflor yellow A in the single and mixed decoction of Radix Angelicae Sinensis and Flos Carthami, and to explore the characteristics of Chinese herbs compatibility. Methods A RP - HPLC was performed on a Phenomenex Cls column （4.6 mm×150 mm, 5 μm） at 30 ℃. The mobile phase consisted of methanol -0.05 % phosphate solution （25 ： 75 V/ V, pH 2.45） at the flow rate of 1.0 mL/min. The automatic sample injector was set at 5 ℃ and the ultraviolet detector was operated at 320 nm for ferulic acid and 403 nm for hydroxysafflor yellow A. Results The linearity ranges of ferulic acid was in the range of 0. 5 - 10.0 μg/mL（ Y = 3. 8372X ＋ 0. 0219, r = 0. 999 8）, the average recoveries of adding sample were from 92.68 % to 101.13 % , the RSD （ n = 6） were less than 3.2 %. The linearity ranges of hydroxysafflor yellow A was in the range of 5.0 - 100.0 μg/mL （ Y = 1. 3647 X - 0. 3714, r = 0. 999 9）, the average recoveries of adding sample were from 100. 17 % to 102.97 % , the RSD （ n = 6） were less than 2. 8 %. The content of ferulic acid in the mixed decoction was 142.64 % higher than that in the single decoction of Radix Angelicae Sinensis, and hydroxysafflor yellow A content was 145.37 % higher than that in the single decoction of Flos Carthami. Conclusion The content of ferulic acid and hydroxysafflor yellow A in mixed decoction is higher than the single decoction of Radix Angelicae Sinensisand and Flos Carthami. The method is simple, fast and accurate. It can he used for the quality control of ferulic acid and hydroxysafflor yellow A simultaneously in extracts of medicinal material.

关 键 词：当归 红花 阿魏酸 羟基红花黄色素A 单煎 共煎 反相高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]