甘肃产秦艽类药材中龙胆苦苷的含量测定  

Determination of Gentiopicroside in Radix Gentianae Macrophyllae Produced in Gansu Province by HPLC

作　　者：马潇[1] 朱俊儒[1] 何禄仁[1] 赵建邦[1] 

机构地区：[1]甘肃省药品检验所,兰州730000

出　　处：《中药新药与临床药理》2009年第5期465-467,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：甘肃省中医药科研项目(No.05-GZK-16)

摘　　要：目的测定甘肃不同产地、不同品种秦艽类药材中活性成分龙胆苦苷的含量。方法采用高效液相色谱法。色谱柱:WatersC18色谱柱;流动相:甲醇-水(3∶7);流速:1.0mL/min;检测波长:254nm;柱温:室温。结果龙胆苦苷在1.7352～17.3520μg范围内具有良好线性关系,平均回收率为97.06%R(SD=2.5%)。结论甘肃产秦艽类药材中龙胆苦苷含量均高于中国药典标准,有一定的开发利用价值。Objective To determine the content of gentiopicroside and to evaluate the quality of Radix Gentianae Macrophyllae in Gansu province. Methods The HPLC method was performed on Waters C18 column. Methanol - water （3 ： 7） were used as the mobile phase, the flow rate was at 1.0 mL ·min^-1 with the detection wavelength being 254 nm. Results For gentiopicroside, the linear range was 1. 7352 - 17. 3520 μg and the average recovery was 97.06 % with RSD =2.5 %. Conclusion The content of gentiopicroside in all of the samples produced in Gansu province is higher than or approach to the standard for Radix Gentianae Macrophyllae in Chinese Pharmacopoeia.

关 键 词：秦艽类药材 龙胆苦苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]