高效液相色谱法测定沉积物中的酚类防腐剂  

DETERMINATION OF PHENOLIC PRESERVATIVES IN SEDIMENTS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：包艳[1] 牛增元 叶曦雯 罗忻 

机构地区：[1]中国科学院青岛生物能源与过程研究所,山东青岛266071 [2]山东出入境检验检疫技术中心,山东青岛266002

出　　处：《海洋湖沼通报》2009年第3期127-136,共10页Transactions of Oceanology and Limnology

基　　金：国家公益性行业科研专项经费(10-66);国家质量监督检验检疫总局科研项目(2006IK092)资助

摘　　要：建立了简便、准确的高效液相色谱法(HPLC)检测沉积物中五氯苯酚(PCP)、四氯苯酚(TeCP)和邻苯基苯酚(OPP)。以甲醇10mmol/L醋酸铵水溶液为流动相进行梯度洗脱,以C18柱为液相分离柱,DAD为检测器,检测波长为220 nm,并且对提取方式、提取溶剂、提取时间进行探索与研究。PCP、TeCP、OPP分析线性范围分别为0.02～200 mg/L(r=0.99994),0.02～200 mg/L(r=0.99990),0.04～200 mg/L(r=0.99995);最低检测浓度均为0.1 ng/g;回收率96.35%～103.6%,RSD≤8.36%(n=7)。本法具有非常高的选择性、灵敏度和准确度,可以分析测定沉积物中的PCP、TeCP、OPP。An analytical method using high performance liquid chromatography （HPLC） was established for simultaneous determination of Pentachlorophenol （PCP）, Tetrachlorophenol （TeCP） and Orthophenylphenol （OPP） in sediments. LC separation was performed on a C18 column with a gradient elution consist of aqueous ammonium acetate and methanol. Analyses were detected using a DAD at the wavelength of 220 nm. The linear range of PCP, TeCP, and OPP were 0.02~200 mg/L （r=0. 99994）, 0.02~200 mg/L （r=0. 99990）, 0.04~200 mg/L （r=0. 99995）, respectively. The detection limits were 0. 1ng/g. The extraction method, extraction solvent, and extraction time were also discussed to give an optimum result. Extraction recoveries were between 96.35%--103.6%, RSDs were below 8.36% （n=7）. The method can be applied to determine PCP, TeCP, and OPP in sediments with satisfactory results.

关 键 词：酚类防腐剂 沉积物 高效液相色谱 测定 

分 类 号：X383[环境科学与工程—环境工程]