龙胆药材提取工艺的正交实验设计法优选  

Orthogonal Experimental Design for Optimization of Extraction of Medicinal Gentian

作　　者：曹悦[1,2] 左代英[2] 王维宁[1] 刘敬武 孙启时[2] 

机构地区：[1]辽宁省食品药品检验所,辽宁沈阳110023 [2]沈阳药科大学,辽宁沈阳110016 [3]辽宁天瑞绿色产业科技开发有限公司,辽宁清原113300

出　　处：《时珍国医国药》2009年第9期2264-2265,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技支撑计划子课题(No.2006BAI06A05-08)

摘　　要：目的建立龙胆最佳提取工艺,为龙胆的种植和质量研究奠定基础。方法采用L9(34)正交表安排实验,确定最佳提取条件。色谱柱为Shimadzu C18(3.0 mm×75 mm,1.7μm);流动相为乙腈-0.4%磷酸(V∶V=5.5∶94.5),流速0.80ml/min;检测波长235,270 nm;柱温为40℃。结果影响因素最大的是提取溶剂,其次是提取方法,最小的是提取时间。结论各因素较优的水平组合为A1B1C1,即甲醇超声提取30 min。Objective To establish best extraction process of Gentiana scabra Bge. by UPLC so as to provide base for the plant and research of Gentiana scabra Bge. Methods By L9 （ 3^4 ） orthogonal design , the optimal condition was confirmed as follows ： Shimadzu C18（3.0 mm ×75 mm,1.7μm）, Acetonitrile -0.1% Phosphorylation（V： V = 5.5： 94.5） as nwbile phase the flow rate was 0.8 ml/min, detection wavelength was 235nm and 270nm and the columne temperature was 40℃. Results The extraction solution was the most effective factor , the second was the method, and the last was the extraction time. Conclusion The best extraction process is A1 B1C1 ： super sonic extraction for 30 min in methanol.

关 键 词：龙胆 正交实验设计法 獐牙菜苦苷 龙胆苦苷 

分 类 号：R284.2[医药卫生—中药学]