灯盏花素与DNA相互作用的电化学及吸收光谱  

Electrochemistry and UV-Vis Spectrometry on Interaction between Breviscapine and DNA

作　　者：郭英[1,2] 贝玉祥[1] 高云涛[1] 

机构地区：[1]云南民族大学化学与生物技术学院,昆明650031 [2]郧阳师范高等专科学校化学系

出　　处：《应用化学》2009年第9期1045-1049,共5页Chinese Journal of Applied Chemistry

基　　金：云南省社会发展科技计划项目(2007B148M)

摘　　要：采用电化学方法研究了灯盏花素与DNA间的相互作用。电化学实验结果表明,DNA的存在使灯盏花素的氧化峰电流减小,且峰电位正移。吸收光谱研究表明,DNA的存在使灯盏花素在335nm处的吸收峰强度降低,呈减色效应,且出现2个等电吸收点,说明灯盏花素与DNA的相互作用以嵌插作用为主。通过计算获得双链DNA(dsDNA)与灯盏花素的结合比为1∶3,结合常数β=3.63×1013。灯盏花素与DNA作用强于其它黄酮类化合物。The interaction between breviscapine and DNA was studied by electrochemical tests. The results show that the oxidation peak current of breviscapine decreased and the peak potential shifted positively in the presence of DNA. The absorption spectrum measurement shows that with the addition of DNA, the intensity of absorption peak of breviscapine at 335 nm decreased, showing hypochromic effect, at the same time, two isoelectric absorption point appeared. The results indicate that breviscapine interacts with DNA mainly intercalation. Calculation indicats that the binding ratio between breviscapine and dsDNA was 1：3, binding constant of 3.63 ×10^13. through with a

关 键 词：灯盏花素 DNA 循环伏安法 吸收光谱 相互作用 

分 类 号：O657.1[理学—分析化学]