广西首次在急性胃肠炎病例中检出GⅠ型诺如病毒  

Detection of genogroup Ⅰ noroviruses from acute gastrocenteritis outpatients in Guangxi for the first time

作　　者：周艳[1] 邓丽丽[1] 刘巍[1] 谭冬梅[1] 唐振柱[1] 杨进业[1] 黄兆勇[1] 杨哲[1] 谢镇国[1] 方志锋[1] 董柏青[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028

出　　处：《中国热带医学》2009年第10期1972-1973,共2页China Tropical Medicine

基　　金：广西区科技厅项目(桂科攻0592007-4;桂科条08-05-01D;桂科攻9732027)

摘　　要：目的研究南宁市某医院门诊急性胃肠炎病例中GⅠ型诺如病毒的感染状况。方法应用荧光定量RT-PCR法对2007年1月～2008年12月南宁市某医院门诊腹泻病例标本进行GⅠ型诺如病毒RNA检测,并对GⅠ型阳性标本进行测序验证。结果在所检测的696份粪便悬液标本中,检出4份GⅠ型阳性,经核酸测序证实为GⅠ型诺如病毒。结论在广西的急性胃肠炎病例中存在GⅠ型诺如病毒感染。Objective To survey the prevalence of Genogroup Ⅰ noroviruses among outpatients with gastroenteritis in Guangxi. Methods Stool samples were collected from diarrhea patients attending outpatient departments of a hospital in Nanning City,between January 2007 and December 2008,and tested for presence of GⅠ norovirus using the real-time quantitative RT-PCR. All positive samples were further sequenced to confirm identities. Results Among the 696 samples,4 were positive for GI noroviruses,all of which were confirmed as GⅠ noroviruses by sequencing. Conclusion There GⅠ norovirus infection is adults with acute gastroenteritis in Guangxi.

关 键 词：诺如病毒 G Ⅰ型 散发病例 荧光定量PCR 

分 类 号：R372.2[医药卫生—病原生物学]