反相高效液相色谱法同时测定复方木香小檗碱片中吴茱萸碱和吴茱萸次碱的含量  

Simultaneous determination of evodiamine and rutacarpine in Fufang Muxiang Xiaobojian tablets by RP-HPLC

作　　者：谢娇[1] 孙丽仁[2] 卢燕香[2] 卢潇潇[1] 

机构地区：[1]萍乡市人民医院药剂科,江西萍乡337055 [2]江西中医学院,南昌330004

出　　处：《中南药学》2009年第9期666-668,共3页Central South Pharmacy

摘　　要：目的建立复方木香小檗碱片中吴茱萸碱与吴茱萸次碱含量测定的方法。方法采用RP-HPLC法，ODS2柱（250mm×4．6mm，5μm），以乙腈-水（39：61）为流动相；流速为1.0mL·min^-1；检测波长为225nm；柱温为35℃；进样量为20μL。结果吴菜萸碱在2．096～10．480μg·mL^-1与峰面积有良好的线性关系，其平均回收率为98．76％，方法精密度（RSD）为1．68％（n=6）；吴茱萸次碱在2．816～14．080μg·mL^-1与峰面积有艮好的线性关系，其平均回收率为99．54％，方法精密度（RSD）为1．93％（n=6）。结论所建立的方法简便易行、准确可靠，可用于复方木香小檗碱片中吴茱萸碱与吴茱萸次碱的含量测定。Objective To establish an RP-HPLC method for simultaneous determination of content of evodiamine and rutacarpine in Fufang Muxiang Xiaobojian tablets. Methods RP-HPLC and ODS2 column （250mm×4.6mm,5μm） were used with acetonitrile-water （39 ： 61） as the mobile phase. The flow rate was 1.0 mL·min^-1. The detection wavelength was 225 nm. The column temperature was 35℃. Results The linear range of evodiamine and rutacarpine was 2. 096-10. 480μg·mL^-1 and 2. 816414. 080μg·mL^-1. The average recovery was 98. 76% and 99.54% with RSD=1. 68%（n=6） and RSD=1.93% （n=6） respectively. Conclusion The method established is sensitive, accurate and can be used for the quality control of Fufang Muxiang Xiaohojian tablets.

关 键 词：复方木香小檗碱片 吴茱萸碱 吴茱萸次碱 HPLC 

分 类 号：R917[医药卫生—药物分析学] R927.1[医药卫生—药学]