高效液相色谱法测定复方羊角颗粒中欧前胡素的含量  

Determination of imperatorin in compound Yangjiao granules by HPLC

作　　者：陈静[1] 谷铁波[1] 陈贵平 

机构地区：[1]湘潭市第一人民医院药剂科,湖南湘潭411101 [2]福寿堂制药有限公司,湖南湘潭411101

出　　处：《中南药学》2009年第9期673-675,共3页Central South Pharmacy

摘　　要：目的建立复方羊角颗粒中欧前胡素的含量测定方法。方法采用高效液相色谱法。色谱柱为Diamonsil C18柱（200mm×4．6mm，5μm）；流动相为甲醇-水（65：35）；检测波长为248mm；流速为1．00mL·min^-1。结果欧前胡素进样量在0．0106～0．0954μg与其峰面积值呈良好的线性关系（r=0．9998），平均加样回收率为99．1％。RSD为0．8％。结论该方法操作简便，结果准确可靠，可用于复方羊角颗粒的质量控制。To determine the content of imperatorin in compound Yangjiao granules by HPLC. Methods The content of imperatorin in compound Yangjiao granules was detemined by HPLC. The column was Diamonsil C18 （200 mm×4.6mm,5μm） ; the mobile phase was methanol-water （65 ： 35） ; the detective wavelength was 248 nm; and the flow rate was 1.00 mL·min^-1. Results The linear range of imperatorin was 0. 010 6-0. 095 4 9g （r=0. 999 8）, the average recovery was 99.1% and RSD was 0.8%. Conclusion The HPLC method is simple, accurate and reliable. It can be used to control the quality of compound Yangjiao granules.

关 键 词：复方羊角颗粒 欧前胡素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R917